ArticleAnalytical chemistry2012
Evaluation of front-end higher energy collision-induced dissociation on a benchtop dual-pressure linear ion trap mass spectrometer for shotgun proteomics.
Article in Analytical chemistry, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed.
- CIDer: A Statistical Framework for Interpreting Differences in CID and HCD Fragmentation.Journal of proteome research · 2021Article
- Discrimination of Isomers of Released N- and O-Glycans Using Diagnostic Product Ions in Negative Ion PGC-LC-ESI-MS/MS.Journal of the American Society for Mass Spectrometry · 2018Article
- Glycoprotein Enrichment Analytical Techniques: Advantages and Disadvantages.Methods in enzymology · 2017Article
- Data-independent-acquisition mass spectrometry for identification of targeted-peptide site-specific modifications.Analytical and bioanalytical chemistry · 2015Article
- Design and performance evaluation of a linear ion trap mass analyzer featuring half round rod electrodes.Journal of the American Society for Mass Spectrometry · 2015Article
- Article
- Implementation of statistical process control for proteomic experiments via LC MS/MS.Journal of the American Society for Mass Spectrometry · 2014Article
- Development and characterization of a novel plug and play liquid chromatography-mass spectrometry (LC-MS) source that automates connections between the capillary trap, column, and emitter.Molecular & cellular proteomics : MCP · 2013Article
- Neutron encoded labeling for peptide identification.Analytical chemistry · 2013Article
- Protein analysis by shotgun/bottom-up proteomics.Chemical reviews · 2013Review
- Learning score function parameters for improved spectrum identification in tandem mass spectrometry experiments.Journal of proteome research · 2012Article
Corrections and comments
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Authors and funding
8 authors.
Funding
Abstract
We report the implementation of front-end higher energy collision-induced dissociation (fHCD) on a benchtop dual-pressure linear ion trap. Software and hardware modifications were employed, described in detail vide-infra, to allow isolated ions to undergo collisions with ambient gas molecules in an intermediate multipole (q00) of the instrument. Results comparing the performance of fHCD and resonance excitation collision-induced dissociation (RE-CID) in terms of injection time, total number of scans, efficiency, mass measurement accuracy (MMA), unique peptide identifications, and spectral quality of labile modified peptides are presented. fHCD is approximately 23% as efficient as RE-CID, and depending on the search algorithm, it identifies 6.6% more or 15% less peptides (q < 0.01) from a soluble whole-cell lysate ( Caenorhabditis elegans ) than RE-CID using Mascot or Sequest search algorithms, respectively. fHCD offers a clear advantage for the analysis of phosphorylated and glycosylated (O-GlcNAc) peptides as the average cross-correlation score (XCorr) for spectra using fHCD was statistically greater (p < 0.05) than for spectra collected using RE-CID.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.