ArticleThe Journal of chemical physics2011
Fluorescent protein barrel fluctuations and oxygen diffusion pathways in mCherry.
Article in The Journal of chemical physics, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- Bright and photostable yellow fluorescent proteins for extended imaging.Nature communications · 2025Article
- PEG-mCherry interactions beyond classical macromolecular crowding.Protein science : a publication of the Protein Society · 2025Article
- Use of the mCherry fluorescent protein to optimize the expression of class I lanthipeptides in Escherichia coli.Microbial cell factories · 2023Article
- Design and biofabrication of bacterial living materials with robust and multiplexed biosensing capabilities.Materials today. Bio · 2023Article
- Nitric Oxide Sensing by a Blue Fluorescent Protein.Antioxidants (Basel, Switzerland) · 2022Article
- Nitric oxide biosensor uncovers diminished ferrous iron-dependency of cultured cells adapted to physiological oxygen levels.Redox biology · 2022Article
- Green-Light-Activated Photoreaction via Genetic Hybridization of Far-Red Fluorescent Protein and Silk.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2018Article
- Plasmonic photocatalyst-like fluorescent proteins for generating reactive oxygen species.Nano convergence · 2018Review
- Improving brightness and photostability of green and red fluorescent proteins for live cell imaging and FRET reporting.Scientific reports · 2016Article
- Advances in Imaging Techniques and Genetically Encoded Probes for Photoacoustic Imaging.Theranostics · 2016Review
- Use of the mCherry Fluorescent Protein To Study Intestinal Colonization by Enterococcus mundtii ST4SA and Lactobacillus plantarum 423 in Mice.Applied and environmental microbiology · 2015Article
- Microfluidics-based selection of red-fluorescent proteins with decreased rates of photobleaching.Integrative biology : quantitative biosciences from nano to macro · 2015Article
- Reporter systems for in vivo tracking of lactic acid bacteria in animal model studies.Gut microbes · 2015Review
- Chromophore photoreduction in red fluorescent proteins is responsible for bleaching and phototoxicity.The journal of physical chemistry. B · 2014Article
- Exploring the diffusion of molecular oxygen in the red fluorescent protein mCherry using explicit oxygen molecular dynamics simulations.The journal of physical chemistry. B · 2013Article
- Exploring structural and optical properties of fluorescent proteins by squeezing: modeling high-pressure effects on the mStrawberry and mCherry red fluorescent proteins.The journal of physical chemistry. B · 2012Article
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
Fluorescent proteins (FPs) are valuable tools as biochemical markers for studying cellular processes. Red fluorescent proteins (RFPs) are highly desirable for in vivo applications because they absorb and emit light in the red region of the spectrum where cellular autofluorescence is low. The naturally occurring fluorescent proteins with emission peaks in this region of the spectrum occur in dimeric or tetrameric forms. The development of mutant monomeric variants of RFPs has resulted in several novel FPs known as mFruits. Though oxygen is required for maturation of the chromophore, it is known that photobleaching of FPs is oxygen sensitive, and oxygen-free conditions result in improved photostabilities. Therefore, understanding oxygen diffusion pathways in FPs is important for both photostabilites and maturation of the chromophores. In this paper, we use molecular dynamics calculations to investigate the protein barrel fluctuations in mCherry, which is one of the most useful monomeric mFruit variant. We employ implicit ligand sampling to determine oxygen pathways from the bulk solvent into the mCherry chromophore in the interior of the protein. We also show that these pathways can be blocked or altered and barrel fluctuations can be reduced by strategic amino acid substitutions.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.