Evidence map›Paper›PMID 22191472›Full record

ArticleBiochemistry2012

Structural analysis of HopPmaL reveals the presence of a second adaptor domain common to the HopAB family of Pseudomonas syringae type III effectors.

Alex U Singer, Bin Wu, Adelinda Yee, Scott Houliston, Xiaohui Xu, Hong Cui, Tatiana Skarina, Maite Garcia, Anthony Semesi, Cheryl H Arrowsmith and 1 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biochemistry, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. Review
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Alex U SingerDepartment of Chemical Engineering and Applied Chemistry, Banting and Best Department of Medical Research, University of Toronto, Toronto, Ontario M5G 1L6, Canada.
Bin Wu
Adelinda Yee
Scott Houliston
Xiaohui Xu
Hong Cui
Tatiana Skarina
Maite Garcia
Anthony Semesi
Cheryl H Arrowsmith
Alexei Savchenko

Funding

The Midwest Center for Structural GenomicsU54GM074942 · NIGMS · UNIVERSITY OF CHICAGO · PI JOACHIMIAK, ANDRZEJ · 2005 to 2009
$51.0M
University of Georgia ProjectU54GM094597 · NIGMS · RUTGERS, THE STATE UNIV OF N.J. · PI ROST, BURKHARD · 2010 to 2014
$36.9M
CIHRNIGMS NIH HHS GM074942NIGMS NIH HHS U54 GM074942NIGMS NIH HHS U54 GM094597
6 · The paper itself

Abstract

HopPmaL is a member of the HopAB family of type III effectors present in the phytopathogen Pseudomonas syringae. Using both X-ray crystallography and solution nuclear magnetic resonance, we demonstrate that HopPmaL contains two structurally homologous yet functionally distinct domains. The N-terminal domain corresponds to the previously described Pto-binding domain, while the previously uncharacterised C-terminal domain spans residues 308-385. While structurally similar, these domains do not share significant sequence similarity and most importantly demonstrate significant differences in key residues involved in host protein recognition, suggesting that each of them targets a different host protein.

Indexed as

Bacterial ProteinsConserved SequenceCrystallography, X-RayHydrophobic and Hydrophilic InteractionsMultigene FamilyPeptide FragmentsPlant DiseasesPlant ProteinsProtein BindingProtein FoldingProtein Serine-Threonine KinasesProtein Structure, TertiaryPseudomonas syringaeSequence AlignmentSolanum lycopersicumavrPto protein, Pseudomonas syringaeBacterial ProteinsPeptide FragmentsPlant ProteinsProtein Serine-Threonine KinasesPto protein, Lycopersicon

Identifiers

PMID22191472
PMCPMC3656468

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.