ArticleRNA (New York, N.Y.)2012
Visualizing large RNA molecules in solution.
Article in RNA (New York, N.Y.), 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 71 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
71 citing papers in PubMed.
- Packaging of Single-Stranded RNA in Viruses and Virus-Like Particles.Annual review of biochemistry · 2026Review
- Nanoscale domains govern local diffusion and ageing within fused-in-sarcoma condensates.Nature nanotechnology · 2026Article
- Article
- Cyclic Macromolecular Chains Visualized by Cryo-EM and AFM Reveal a Ring Expansion Polymerization Mechanism in a Classical Synthetic Pathway to Polyphosphazenes.ACS macro letters · 2025Article
- Tuning hydrogen bonds and electrostatics with convection for purifying mRNA: A paradigm shift.Science advances · 2025Article
- Virus-Mimicking Polymer Nanocomplexes Co-Assembling HCV E1E2 and Core Proteins with TLR 7/8 Agonist-Synthesis, Characterization, and In Vivo Activity.Journal of functional biomaterials · 2025Article
- How much genetic information in RNA form can be protected by a CCMV virus-like particle?PloS one · 2025Article
- Nanoscale cellular organization of viral RNA and proteins in SARS-CoV-2 replication organelles.Nature communications · 2024Article
- Article
- Nanoscale cellular organization of viral RNA and proteins in SARS-CoV-2 replication organelles.bioRxiv : the preprint server for biology · 2024Article
- Directly visualizing individual polyorganophosphazenes and their single-chain complexes with proteins.Communications materials · 2024Article
- Cryo-EM and AFM visualize linear polyorganophosphazene: individual chains and single-chain assemblies with proteins.Research square · 2023Article
- Sulfonylation of RNA 2'-OH groups.ACS central science · 2023Article
- Partitioning of ribonucleoprotein complexes from the cellular actin cortex.Science advances · 2022Article
- Acylation probing of "generic" RNA libraries reveals critical influence of loop constraints on reactivity.Cell chemical biology · 2022Article
- Differences in ion-RNA binding modes due to charge density variations explain the stability of RNA in monovalent salts.Science advances · 2022Article
- A Biopharmaceutical Perspective on Higher-Order Structure and Thermal Stability of mRNA Vaccines.Molecular pharmaceutics · 2022Review
- What are the distinguishing features and size requirements of biomolecular condensates and their implications for RNA-containing condensates?RNA (New York, N.Y.) · 2022Review
- An integrative NMR-SAXS approach for structural determination of large RNAs defines the substrate-free state of a trans-cleaving Neurospora Varkud Satellite ribozyme.Nucleic acids research · 2021Article
- Article
11 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
Abstract
Single-stranded RNAs (ssRNAs) longer than a few hundred nucleotides do not have a unique structure in solution. Their equilibrium properties therefore reflect the average of an ensemble of structures. We use cryo-electron microscopy to image projections of individual long ssRNA molecules and characterize the anisotropy of their ensembles in solution. A flattened prolate volume is found to best represent the shapes of these ensembles. The measured sizes and anisotropies are in good agreement with complementary determinations using small-angle X-ray scattering and coarse-grained molecular dynamics simulations. A long viral ssRNA is compared with shorter noncoding transcripts to demonstrate that prolate geometry and flatness are generic properties independent of sequence length and origin. The anisotropy persists under physiological as well as low-ionic-strength conditions, revealing a direct correlation between secondary structure asymmetry and 3D shape and size. We discuss the physical origin of the generic anisotropy and its biological implications.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.