ArticleMolecular biology of the cell2012
The mechanisms of microtubule catastrophe and rescue: implications from analysis of a dimer-scale computational model.
Article in Molecular biology of the cell, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 60 papers.
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Who cites it
60 citing papers in PubMed.
- Single-molecule microtubule dynamics measurements reveal an intermediate state and clarify the role of nucleotide.Nature communications · 2025Article
- Mechanisms of microtubule dynamics from single-molecule measurements.bioRxiv : the preprint server for biology · 2025Article
- EB3-informed dynamics of the microtubule stabilizing cap during stalled growth.Biophysical journal · 2025Article
- HURP regulates Kif18A recruitment and activity to synergistically control microtubule dynamics.Nature communications · 2024Article
- Molecular interplay between HURP and Kif18A in mitotic spindle regulation.Research square · 2024Article
- Minimal Mechanisms of Microtubule Length Regulation in Living Cells.Bulletin of mathematical biology · 2024Article
- Rapid binding to protofilament edge sites facilitates tip tracking of EB1 at growing microtubule plus-ends.eLife · 2024Article
- Article
- Interface-acting nucleotide controls polymerization dynamics at microtubule plus- and minus-ends.eLife · 2024Article
- Measuring and modeling forces generated by microtubules.Biophysical reviews · 2023Review
- Beyond the GTP-cap: Elucidating the molecular mechanisms of microtubule catastrophe.BioEssays : news and reviews in molecular, cellular and developmental biology · 2023Article
- Theory of tip structure-dependent microtubule catastrophes and damage-induced microtubule rescues.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Article
- Bending-torsional elasticity and energetics of the plus-end microtubule tip.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Quantification of microtubule stutters: dynamic instability behaviors that are strongly associated with catastrophe.Molecular biology of the cell · 2022Article
- Effects of random hydrolysis on biofilament length distributions in a shared subunit pool.Biophysical journal · 2022Article
- Effects of length-dependent positive feedback on length distributions of microtubules undergoing hydrolysis.Journal of biosciences · 2022Article
- Regulation of microtubule dynamics, mechanics and function through the growing tip.Nature reviews. Molecular cell biology · 2021Review
- XMAP215 promotes microtubule catastrophe by disrupting the growing microtubule end.The Journal of cell biology · 2021Article
- Chemically Fueled Self-Assembly in Biology and Chemistry.Angewandte Chemie (International ed. in English) · 2021Review
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Authors and funding
7 authors.
Funding
Abstract
Microtubule (MT) dynamic instability is fundamental to many cell functions, but its mechanism remains poorly understood, in part because it is difficult to gain information about the dimer-scale events at the MT tip. To address this issue, we used a dimer-scale computational model of MT assembly that is consistent with tubulin structure and biochemistry, displays dynamic instability, and covers experimentally relevant spans of time. It allows us to correlate macroscopic behaviors (dynamic instability parameters) with microscopic structures (tip conformations) and examine protofilament structure as the tip spontaneously progresses through both catastrophe and rescue. The model's behavior suggests that several commonly held assumptions about MT dynamics should be reconsidered. Moreover, it predicts that short, interprotofilament "cracks" (laterally unbonded regions between protofilaments) exist even at the tips of growing MTs and that rapid fluctuations in the depths of these cracks influence both catastrophe and rescue. We conclude that experimentally observed microtubule behavior can best be explained by a "stochastic cap" model in which tubulin subunits hydrolyze GTP according to a first-order reaction after they are incorporated into the lattice; catastrophe and rescue result from stochastic fluctuations in the size, shape, and extent of lateral bonding of the cap.
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