ArticleMolecular biology of the cell2012
Analysis of yeast endocytic site formation and maturation through a regulatory transition point.
Article in Molecular biology of the cell, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 58 papers.
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Who cites it
58 citing papers in PubMed, 98 citations in OpenAlex.
- The complex subunit VdAP-2α regulates polar growth, ergosterol metabolism, and virulence inVirulence · 2026Article
- Polarized anionic phospholipids and exocytosis are implicated in the polarized recruitment of budding yeast AP180, an endocytic initiator.Molecular biology of the cell · 2026Article
- Endocytosis and trans-Golgi Network in Yeast.Sub-cellular biochemistry · 2026Review
- Beyond Clathrin: Decoding the Mechanism of Ultrafast Endocytosis.Physiology (Bethesda, Md.) · 2025Review
- Cigarette smoke impairs the endocytotic process inToxicology research · 2025Article
- Candida albicans Ssy1 is the extracellular sensor of gut microbiota-derived peptidoglycan fragments mediating invasive hyphal growth in the host.Nature communications · 2025Article
- The conserved protein adaptors CALM/AP180 and FCHo1/2 cooperatively recruit Eps15 to promote the initiation of clathrin-mediated endocytosis in yeast.PLoS biology · 2024Article
- Mechanistic divergences of endocytic clathrin-coated vesicle formation in mammals, yeasts and plants.Journal of cell science · 2024Review
- The EH domain-containing protein, EdeA, is involved in endocytosis, cell wall integrity, and pathogenicity inmSphere · 2024Article
- Proteasome assembly chaperone translation upon stress requires Ede1 phase separation at the plasma membrane.iScience · 2024Article
- LIRcentral: a manually curated online database of experimentally validated functional LIR motifs.Autophagy · 2023Article
- Protein condensates as flexible platforms for membrane traffic.Current opinion in cell biology · 2023Review
- The Early Endocytosis GeneJournal of fungi (Basel, Switzerland) · 2023Article
- Light-dependent inhibition of clathrin-mediated endocytosis in yeast unveils conserved functions of the AP2 complex.iScience · 2023Article
- Load adaptation by endocytic actin networks.Molecular biology of the cell · 2022Article
- Article
- Alpha-arrestins Aly1/Art6 and Aly2/Art3 regulate trafficking of the glycerophosphoinositol transporter Git1 and impact phospholipid homeostasis.Biology of the cell · 2022Article
- Endocytic protein Pal1 regulates appressorium formation and is required for full virulence of Magnaporthe oryzae.Molecular plant pathology · 2022Article
- Article
- Candida albicansmSphere · 2021Article
Corrections and comments
- Erratum issued
Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
The earliest stages of endocytic site formation and the regulation of endocytic site maturation are not well understood. Here we analyzed the order in which the earliest proteins are detectable at endocytic sites in budding yeast and found that an uncharacterized protein, Pal1p/Ydr348cp, is also present at the initial stages of endocytosis. Because Ede1p (homologue of Eps15) and clathrin are the early-arriving proteins most important for cargo uptake, their roles during the early stages of endocytosis were examined more comprehensively. Ede1p is necessary for efficient recruitment of most early-arriving proteins, but not for the recruitment of the adaptor protein Yap1802p, to endocytic sites. The early-arriving proteins, as well as the later-arriving proteins Sla2p and Ent1/2p (homologues of Hip1R and epsins), were found to have longer lifetimes in CLC1-knockout yeast, which indicates that clathrin light chain facilitates the transition from the intermediate to late coat stages. Cargo also arrives during the early stages of endocytosis, and therefore its effect on endocytic machinery dynamics was investigated. Our results are consistent with a role for cargo in regulating the transition of endocytic sites from the early stages of formation to the late stages during which vesicle formation occurs.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.