ArticleJournal of assisted reproduction and genetics2012
In vitro follicle growth under non-attachment conditions and decreased FSH levels reduces Lhcgr expression in cumulus cells and promotes oocyte developmental competence.
Article in Journal of assisted reproduction and genetics, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 22 citations in OpenAlex.
- A matrix-free 3D in vitro follicle culture system in mice exhibits enhanced oocyte meiotic and developmental competence compared to hydrogel encapsulation.Molecular human reproduction · 2025Article
- Ovarian Mechanobiology: Understanding the Interplay Between Mechanics and Follicular Development.Cells · 2025Review
- Pronuclear transfer rescues poor embryo development ofHuman reproduction open · 2024Article
- Glucose and redox metabolism in meiotically blocked in vitro grown mouse antral follicles.Journal of assisted reproduction and genetics · 2023Article
- Alternative Culture Systems for Bovine Oocyte In Vitro Maturation: Liquid Marbles and Differentially Shaped 96-Well Plates.Animals : an open access journal from MDPI · 2023Article
- Oocyte quality following in vitro follicle development†.Biology of reproduction · 2022Review
- Telomere associated gene expression as well as TERT protein level and telomerase activity are altered in the ovarian follicles of aged mice.Scientific reports · 2021Article
- Anti-Müllerian hormone is a survival factor and promotes the growth of rhesus macaque preantral follicles during matrix-free culture.Biology of reproduction · 2018Article
- Article
- Engineering the ovarian cycle using in vitro follicle culture.Human reproduction (Oxford, England) · 2015Article
- Three-dimensional systems for in vitro follicular culture: overview of alginate-based matrices.Reproduction, fertility, and development · 2014Review
- FSH in vitro versus LH in vivo: similar genomic effects on the cumulus.Journal of ovarian research · 2013Article
- Quality and functionality of human ovarian tissue after cryopreservation using an original slow freezing procedure.Journal of assisted reproduction and genetics · 2013Article
- TheJournal of reproduction & infertilityArticle
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
purposeThe in-vitro environment influences oocyte competence and gene expression in cumulus cells and oocytes. Effects of culturing under non-attachment conditions and varying follicle exposure to FSH were investigated at the mRNA level and on oocyte developmental capacity.
methodsQuantitative PCR analysis of Gdf9, Mater, Nmp2 (in oocytes), Lhcgr and Amh (in cumulus cells), and oocyte developmental competence after in vitro follicle culture were evaluated.
resultsFollicle survival (98.7%) and polar body rate (94%) were similar for all conditions. Estradiol and progesterone production were significantly lower in non-attachment follicles (10-fold and 3-fold, respectively). Under non-attachment conditions, a higher two-cell rate (69.9%) and total blastocyst yield (48.5%) were obtained and, by decreasing FSH levels during culture, Lhcgr transcripts were significantly reduced to levels similar to in-vivo. Levels of oocyte-specific transcripts were not significantly influenced by in-vitro conditions.
conclusionNon-attachment conditions influence follicle steroid secretory capacity and, together with dynamic FSH doses, positively influence cumulus cell gene expression and oocyte developmental competence.
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