ArticleJournal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research2012
Examination of nuclear receptor expression in osteoblasts reveals Rorβ as an important regulator of osteogenesis.
Article in Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
26 citing papers in PubMed, 1 synthesis or guideline pooled it, 40 citations in OpenAlex.
- Consequences of Aging on Bone.Aging and disease · 2023Pooled it
- New insights into bone pathology through a transcriptomic profiling of an osteoblast model of Gaucher disease.Molecular genetics and metabolism reports · 2026Article
- The retinoic acid-related orphan receptors (RORs) signaling axis in skeletal homeostasis: mechanistic insights and chronotherapeutic prospects.Annals of medicine · 2026Review
- An Acetylation-Primed SUMOylation Switch Controls RORβ Stability through a p300-SIRT1 Regulatory Axis.Research square · 2026Article
- Acetylation-Primed SUMOylation Drives RORβ Turnover via a p300-SIRT1 Regulatory Axis.bioRxiv : the preprint server for biology · 2026Article
- RORβ Deficiency Inhibits Endochondral Ossification in Mice by Mediating the HIF-1α/VEGFA Signaling Pathway.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2025Article
- Estrogen-regulated miRs in bone enhance osteoblast differentiation and matrix mineralization.Molecular therapy. Nucleic acids · 2023Article
- Utilizing Variants Identified with Multiple Genome-Wide Association Study Methods Optimizes Genomic Selection for Growth Traits in Pigs.Animals : an open access journal from MDPI · 2023Article
- The role of retinoic acid receptor-related orphan receptors in skeletal diseases.Frontiers in endocrinology · 2023Review
- Rora Regulates Neutrophil Migration and Activation in Zebrafish.Frontiers in immunology · 2022Article
- Senescent cells exacerbate chronic inflammation and contribute to periodontal disease progression in old mice.Journal of periodontology · 2021Article
- Prostaglandin EStem cell reviews and reports · 2021Article
- Differential processing and localization of human Nocturnin controls metabolism of mRNA and nicotinamide adenine dinucleotide cofactors.The Journal of biological chemistry · 2020Article
- RORB and RORC associate with human islet dysfunction and inhibit insulin secretion in INS-1 cells.Islets · 2019Article
- miR-219a-5p Regulates Rorβ During Osteoblast Differentiation and in Age-related Bone Loss.Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research · 2019Article
- Identification of potent RORβ modulators: Scaffold variation.Bioorganic & medicinal chemistry letters · 2018Article
- Identification of an aminothiazole series of RORβ modulators.Bioorganic & medicinal chemistry letters · 2018Article
- Osteoprotection Through the Deletion of the Transcription Factor Rorβ in Mice.Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research · 2018Article
- Insights into the Role of Circadian Rhythms in Bone Metabolism: A Promising Intervention Target?BioMed research international · 2018Review
- The multi-faceted role of retinoid X receptor in bone remodeling.Cellular and molecular life sciences : CMLS · 2017Review
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
A complex network of transcription factors contributes to the establishment and maintenance of the osteoblastic phenotype. Although relatively few transcription factors, such as Runx2 and osterix, are essential to the process of osteoblastic differentiation, others serve the purpose of fine-tuning in response to various environmental and hormonal cues. The nuclear receptor (NR) superfamily of transcription factors are involved in numerous aspects of bone biology. In this study, we characterized the expression pattern of the entire NR superfamily in differentiating primary murine calvarial cells in order to identify novel NR regulatory patterns. Dynamic patterns of NR expression were observed throughout the differentiation process. Interestingly, retinoic acid receptor-related orphan receptor β (Rorβ) expression was markedly suppressed at later stages of differentiation. To gain further insight into the function of NRs in bone biology, the NR superfamily was also profiled in mouse bone marrow precursor cells isolated from either young (6-month) or aging, osteoporotic (18-22-month) mice. Of interest, Rorβ was potently overexpressed in the aged cohort. Collectively, these data provided evidence that Rorβ expression is inversely correlated with osteogenic potential, suggesting Rorβ may be an important and unexplored regulator of osteogenesis. To validate this hypothesis, a cell model stably expressing Rorβ in mouse osteoblastic MC3T3-E1 cells was produced (MC3T3-Rorβ). These cells displayed markedly suppressed bone nodule formation as well as reduced osteocalcin and osterix gene expression. Because these genes are Runx2 targets, we reasoned that Rorβ may interfere with Runx2 activity. Consistent with this, transient transfection analysis demonstrated that Rorβ inhibited Runx2-dependent activation of a Runx2-reporter construct. In summary, our data provide a comprehensive profile of NR expression during osteoblast differentiation and identify Rorβ as a novel regulator of osteogenesis and potentially of age-related bone loss through antagonism of Runx2 activity.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.