ReviewApplied microbiology and biotechnology2012
The great screen anomaly--a new frontier in product discovery through functional metagenomics.
Review in Applied microbiology and biotechnology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
41 citing papers in PubMed.
- Screening of Marine Bacteria for Lipase Activity and Application as Whole-Cell Biocatalysts.Microorganisms · 2026Article
- Unveiling hidden microbial diversity in Mars 2020 mission assembly cleanrooms with molecular insights into the persistence and perseverance of novel species defying metagenome sequencing.Microbiology spectrum · 2026Article
- Starch and Cellulose Degradation in the Rumen and Applications of Metagenomics on Ruminal Microorganisms.Animals : an open access journal from MDPI · 2022Review
- A novel metagenome-derived viral RNA polymerase and its application in a cell-free expression system for metagenome screening.Scientific reports · 2022Article
- Bioprospecting for Thermozymes and Characterization of a Novel Lipolytic Thermozyme Belonging to the SGNH/GDSL Family of Hydrolases.International journal of molecular sciences · 2022Article
- Secondary metabolite biosynthetic diversity in Arctic Ocean metagenomes.Microbial genomics · 2021Article
- The Link Between the Ecology of the Prokaryotic Rare Biosphere and Its Biotechnological Potential.Frontiers in microbiology · 2020Review
- Novel Genes Involved in Resistance to Both Ultraviolet Radiation and Perchlorate From the Metagenomes of Hypersaline Environments.Frontiers in microbiology · 2020Article
- Recovery and functional validation of hidden soil enzymes in metagenomic libraries.MicrobiologyOpen · 2019Article
- Dual-barcoded shotgun expression library sequencing for high-throughput characterization of functional traits in bacteria.Nature communications · 2019Article
- The art of vector engineering: towards the construction of next-generation genetic tools.Microbial biotechnology · 2019Review
- Diverse and Abundant Secondary Metabolism Biosynthetic Gene Clusters in the Genomes of Marine Sponge Derived Streptomyces spp. Isolates.Marine drugs · 2018Article
- Article
- Mining Novel Constitutive Promoter Elements in Soil Metagenomic Libraries inFrontiers in microbiology · 2018Article
- Production and characterization of a novel antifungal chitinase identified by functional screening of a suppressive-soil metagenome.Microbial cell factories · 2017Article
- Buwchitin: A Ruminal Peptide with Antimicrobial Potential againstFrontiers in chemistry · 2017Article
- Different inocula produce distinctive microbial consortia with similar lignocellulose degradation capacity.Applied microbiology and biotechnology · 2016Article
- Discovery Strategies of Bioactive Compounds Synthesized by Nonribosomal Peptide Synthetases and Type-I Polyketide Synthases Derived from Marine Microbiomes.Marine drugs · 2016Review
- Assessing the impact of long term frozen storage of faecal samples on protein concentration and protease activity.Journal of microbiological methods · 2016Article
- Use of Substrate-Induced Gene Expression in Metagenomic Analysis of an Aromatic Hydrocarbon-Contaminated Soil.Applied and environmental microbiology · 2016Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Functional metagenomics, the study of the collective genome of a microbial community by expressing it in a foreign host, is an emerging field in biotechnology. Over the past years, the possibility of novel product discovery through metagenomics has developed rapidly. Thus, metagenomics has been heralded as a promising mining strategy of resources for the biotechnological and pharmaceutical industry. However, in spite of innovative work in the field of functional genomics in recent years, yields from function-based metagenomics studies still fall short of producing significant amounts of new products that are valuable for biotechnological processes. Thus, a new set of strategies is required with respect to fostering gene expression in comparison to the traditional work. These new strategies should address a major issue, that is, how to successfully express a set of unknown genes of unknown origin in a foreign host in high throughput. This article is an opinionating review of functional metagenomic screening of natural microbial communities, with a focus on the optimization of new product discovery. It first summarizes current major bottlenecks in functional metagenomics and then provides an overview of the general metagenomic assessment strategies, with a focus on the challenges that are met in the screening for, and selection of, target genes in metagenomic libraries. To identify possible screening limitations, strategies to achieve optimal gene expression are reviewed, examining the molecular events all the way from the transcription level through to the secretion of the target gene product.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.