ArticleJournal of clinical microbiology2012
Development of specific sequence-characterized amplified region markers for detecting Histoplasma capsulatum in clinical and environmental samples.
Article in Journal of clinical microbiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 31 citations in OpenAlex.
- Disseminated histoplasmosis from western Mexico-rethinking our geographic distribution of endemic fungal species: a case report and review of literature.Journal of medical case reports · 2024Review
- Central Nervous System Histoplasmosis: An Updated Insight.Pathogens (Basel, Switzerland) · 2023Article
- Diagnostic accuracy of antigen detection in urine and molecular assays testing in different clinical samples for the diagnosis of progressive disseminated histoplasmosis in patients living with HIV/AIDS: A prospective multicenter study in Mexico.PLoS neglected tropical diseases · 2021Article
- Diagnosis of histoplasmosis: current status and perspectives.Applied microbiology and biotechnology · 2021Review
- The global epidemiology of emergingStudies in mycology · 2020Article
- The Role of Molecular Tests in the Diagnosis of Disseminated Histoplasmosis.Journal of fungi (Basel, Switzerland) · 2019Review
- Molecular epidemiology of ColombianHeliyon · 2019Article
- Detection ofThe American journal of tropical medicine and hygiene · 2018Article
- Usefulness of molecular markers in the diagnosis of occupational and recreational histoplasmosis outbreaks.Folia microbiologica · 2017Article
- Detection and phylogenetic characterization of a case of Histoplasma capsulatum infection in mainland China.The American journal of tropical medicine and hygiene · 2014Article
- A multiplex real-time PCR assay for identification of Pneumocystis jirovecii, Histoplasma capsulatum, and Cryptococcus neoformans/Cryptococcus gattii in samples from AIDS patients with opportunistic pneumonia.Journal of clinical microbiology · 2014Article
- Histoplasma capsulatum and Pneumocystis spp. co-infection in wild bats from Argentina, French Guyana, and Mexico.BMC microbiology · 2014Article
- Design and evaluation of an AFLP molecular marker for the detection of Coccidioides spp. in biological samples.The Brazilian journal of infectious diseases : an official publication of the Brazilian Society of Infectious DiseasesArticle
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Sequence-characterized amplified region (SCAR) markers, generated by randomly amplified polymorphic DNA (RAPD)-PCR, were developed to detect Histoplasma capsulatum selectively in clinical and environmental samples. A 1,200-bp RAPD-PCR-specific band produced with the 1281-1283 primers was cloned, sequenced, and used to design two SCAR markers, 1281-1283(220) and 1281-1283(230). The specificity of these markers was confirmed by Southern hybridization. To evaluate the relevance of the SCAR markers for the diagnosis of histoplasmosis, another molecular marker (M antigen probe) was used for comparison. To validate 1281-1283(220) and 1281-1283(230) as new tools for the identification of H. capsulatum, the specificity and sensitivity of these markers were assessed for the detection of the pathogen in 36 clinical (17 humans, as well as 9 experimentally and 10 naturally infected nonhuman mammals) and 20 environmental (10 contaminated soil and 10 guano) samples. Although the two SCAR markers and the M antigen probe identified H. capsulatum isolates from different geographic origins in America, the 1281-1283(220) SCAR marker was the most specific and detected the pathogen in all samples tested. In contrast, the 1281-1283(230) SCAR marker and the M antigen probe also amplified DNA from Aspergillus niger and Cryptococcus neoformans, respectively. Both SCAR markers detected as little as 0.001 ng of H. capsulatum DNA, while the M antigen probe detected 0.5 ng of fungal DNA. The SCAR markers revealed the fungal presence better than the M antigen probe in contaminated soil and guano samples. Based on our results, the 1281-1283(220) marker can be used to detect and identify H. capsulatum in samples from different sources.
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