ArticleJournal of clinical microbiology2012
Genetic features differentiating bovine, food, and human isolates of shiga toxin-producing Escherichia coli O157 in The Netherlands.
Article in Journal of clinical microbiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
- Article
- Genomic Epidemiology of Shiga Toxin-ProducingAnimals : an open access journal from MDPI · 2021Article
- Farm animal contact is associated with progression to Hemolytic uremic syndrome in patients with Shiga toxin-producingOne health (Amsterdam, Netherlands) · 2020Article
- Genotypic Features of Clinical and BovineMicroorganisms · 2018Review
- Comparative Genomic Analysis of Escherichia coli O157:H7 Isolated from Super-Shedder and Low-Shedder Cattle.PloS one · 2016Article
- Diversity of Survival Patterns among Escherichia coli O157:H7 Genotypes Subjected to Food-Related Stress Conditions.Frontiers in microbiology · 2016Article
- First step in using molecular data for microbial food safety risk assessment; hazard identification of Escherichia coli O157:H7 by coupling genomic data with in vitro adherence to human epithelial cells.International journal of food microbiology · 2015Article
- Whole Genome Sequencing demonstrates that Geographic Variation of Escherichia coli O157 Genotypes Dominates Host Association.Scientific reports · 2015Article
- Multilocus genotypic characterization of Escherichia coli O157:H7 recovered from food sources.Epidemiology and infection · 2015Article
- Prevalence of carriage of Shiga toxin-producing Escherichia coli serotypes O157:H7, O26:H11, O103:H2, O111:H8, and O145:H28 among slaughtered adult cattle in France.Applied and environmental microbiology · 2015Article
- Geographically distinct Escherichia coli O157 isolates differ by lineage, Shiga toxin genotype, and total shiga toxin production.Journal of clinical microbiology · 2015Article
- Article
- Molecular typing of Escherichia coli O157:H7 isolates from Swedish cattle and human cases: population dynamics and virulence.Journal of clinical microbiology · 2014Article
- Predicting the public health benefit of vaccinating cattle against Escherichia coli O157.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Multilocus genotype analysis of Escherichia coli O157 isolates from Australia and the United States provides evidence of geographic divergence.Applied and environmental microbiology · 2013Article
- Characterization of Escherichia coli O157:H7 strains isolated from supershedding cattle.Applied and environmental microbiology · 2013Article
- Molecular and antimicrobial susceptibility analyses distinguish clinical from bovine Escherichia coli O157 strains.Journal of clinical microbiology · 2013Article
- Characteristics of clinical Shiga toxin-producing Escherichia coli isolated from British Columbia.BioMed research international · 2013Article
- Phylogenetically related Argentinean and Australian Escherichia coli O157 isolates are distinguished by virulence clades and alternative Shiga toxin 1 and 2 prophages.Applied and environmental microbiology · 2012Article
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8 authors.
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Abstract
The frequency of Escherichia coli O157 genotypes among bovine, food, and human clinical isolates from The Netherlands was studied. Genotyping included the lineage-specific polymorphism assay (LSPA6), the Shiga-toxin-encoding bacteriophage insertion site assay (SBI), and PCR detection and/or subtyping of virulence factors and markers [stx1, stx(2a)/stx(2c), q21/Q933, tir(A255T), and rhsA(C3468G)]. LSPA6 lineage II dominated among bovine isolates (63%), followed by lineage I/II (35.6%) and lineage I (1.4%). In contrast, the majority of the human isolates were typed as lineage I/II (77.6%), followed by lineage I (14.1%) and lineage II (8.2%). Multivariate analysis revealed that the tir(A255T) SNP and the stx(2a)/stx(2c) gene variants were the genetic features most differentiating human from bovine isolates. Bovine and food isolates were dominated by stx(2c) (86.4% and 65.5%, respectively). Among human isolates, the frequency of stx(2c) was 36.5%, while the frequencies of stx(2a) and stx(2a) plus stx(2c) were 41.2% and 22.4%, respectively. Bovine isolates showed equal distribution of tir(255A) (54.8%) and tir(255T) (45.2%), while human isolates were dominated by the tir(255T) genotype (92.9%). LSPA6 lineage I isolates were all genotype stx(2c) and tir(255T), while LSPA6 lineage II was dominated by tir(255A) (86.4%) and stx(2c) (90.9%). LSPA6 lineage I/II isolates were all genotype tir(255T) but showed more variation in stx(2) types. The results support the hypothesis that in The Netherlands, the genotypes primarily associated with human disease form a minor subpopulation in the bovine reservoir. Comparison with published data revealed that the distribution of LSPA6 lineages among bovine and human clinical isolates differs considerably between The Netherlands and North America.
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