ArticleNucleic acids research2012
RNA aptamers that functionally interact with green fluorescent protein and its derivatives.
Article in Nucleic acids research, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
31 citing papers in PubMed, 57 citations in OpenAlex.
- Expression of nano-engineered RNA organelles in bacteria.Nature communications · 2026Article
- Orthogonal RNA-regulated destabilization domains for three-color RNA imaging with minimal RNA perturbation.Nature methods · 2026Article
- Green Fluorescent Protein SELEX: Immobilization Chemistry and His-Tag Epitope Bias.Angewandte Chemie (International ed. in English) · 2025Article
- Super-Resolved Protein Imaging Using Bifunctional Light-Up Aptamers.Angewandte Chemie (International ed. in English) · 2024Article
- Co-transcriptional production of programmable RNA condensates and synthetic organelles.Nature nanotechnology · 2024Article
- Recent Advances in Aptamer-Based Biosensors for Bacterial Detection.Biosensors · 2024Review
- Structural Insights into the Binding of Red Fluorescent Protein mCherry-Specific Nanobodies.International journal of molecular sciences · 2023Article
- Article
- Optimization of Short RNA Aptamers for TNBC Cell Targeting.International journal of molecular sciences · 2022Article
- A Molecular Communications System for the Detection of Inflammatory Levels Related to COVID-19 Disease.IEEE transactions on molecular, biological, and multi-scale communications · 2021Article
- High-Throughput Selection and Characterisation of Aptamers on Optical Next-Generation Sequencers.International journal of molecular sciences · 2021Review
- DNA origami signposts for identifying proteins on cell membranes by electron cryotomography.Cell · 2021Article
- Modular and Versatile Trans-Encoded Genetic Switches.Angewandte Chemie (International ed. in English) · 2020Article
- RNA aptamer capture of macromolecular complexes for mass spectrometry analysis.Nucleic acids research · 2020Article
- Reporter-recruiting bifunctional aptasensor for bioluminescent analytical assays.RSC advances · 2020Article
- Imaging mRNA trafficking in living cells using fluorogenic proteins.Current opinion in chemical biology · 2020Review
- Unraveling Determinants of Affinity Enhancement in Dimeric Aptamers for a Dimeric Protein.Scientific reports · 2019Article
- Linking RNA Sequence, Structure, and Function on Massively Parallel High-Throughput Sequencers.Cold Spring Harbor perspectives in biology · 2019Review
- Key Aspects of Nucleic Acid Library Design for in Vitro Selection.International journal of molecular sciences · 2018Review
- Multivalent Aptamers: Versatile Tools for Diagnostic and Therapeutic Applications.Molecules (Basel, Switzerland) · 2016Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 4 institutions in 2 countries.
Funding
Abstract
Green Fluorescent Protein (GFP) and related fluorescent proteins (FPs) have been widely used to tag proteins, allowing their expression and subcellular localization to be examined in real time in living cells and animals. Similar fluorescent methods are highly desirable to detect and track RNA and other biological molecules in living cells. For this purpose, we have developed a group of RNA aptamers that bind GFP and related proteins, which we term Fluorescent Protein-Binding Aptamers (FPBA). These aptamers bind GFP, YFP and CFP with low nanomolar affinity and binding decreases GFP fluorescence, whereas slightly augmenting YFP and CFP brightness. Aptamer binding results in an increase in the pKa of EGFP, decreasing the 475 nm excited green fluorescence at a given pH. We report the secondary structure of FPBA and the ability to synthesize functional multivalent dendrimers. FPBA expressed in live cells decreased GFP fluorescence in a valency-dependent manner, indicating that the RNA aptamers function within cells. The development of aptamers that bind fluorescent proteins with high affinity and alter their function, markedly expands their use in the study of biological pathways.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.