ArticleNanomedicine (London, England)2012
Nanoliter droplet vitrification for oocyte cryopreservation.
Article in Nanomedicine (London, England), 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Advanced technologies for the preservation of mammalian biospecimens.Nature biomedical engineering · 2021Review
- [Experimental study on high throughput vitrification by micro-droplet spray method].Sheng wu yi xue gong cheng xue za zhi = Journal of biomedical engineering = Shengwu yixue gongchengxue zazhi · 2019Article
- Cryoprotectant-free cryopreservation of mammalian cells by superflash freezing.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Bio-inspired solute enables preservation of human oocytes using minimum volume vitrification.Journal of tissue engineering and regenerative medicine · 2018Article
- Hydrogel Encapsulation Facilitates Rapid-Cooling Cryopreservation of Stem Cell-Laden Core-Shell Microcapsules as Cell-Biomaterial Constructs.Advanced healthcare materials · 2017Article
- High-Throughput Non-Contact Vitrification of Cell-Laden Droplets Based on Cell Printing.Scientific reports · 2015Article
- Deformation of a single mouse oocyte in a constricted microfluidic channel.Microfluidics and nanofluidics · 2015Article
- Preserving human cells for regenerative, reproductive, and transfusion medicine.Biotechnology journal · 2014Review
- Manipulating biological agents and cells in micro-scale volumes for applications in medicine.Chemical Society reviews · 2013Review
- Bioprinting: Functional droplet networks.Nature materials · 2013Article
- Bioprinting for stem cell research.Trends in biotechnology · 2013Review
- Integrating nanoscale technologies with cryogenics: a step towards improved biopreservation.Nanomedicine (London, England) · 2012Article
- Emerging technologies for assembly of microscale hydrogels.Advanced healthcare materials · 2012Review
- Microfluidics for cryopreservation.Biotechnology advancesReview
Corrections and comments
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Authors and funding
12 authors.
Funding
Abstract
aimOocyte cryopreservation remains largely experimental, with live birth rates of only 2-4% per thawed oocyte. In this study, we present a nanoliter droplet technology for oocyte vitrification. MATERIALS &
methodsAn ejector-based droplet vitrification system was designed to continuously cryopreserve oocytes in nanoliter droplets. Oocyte survival rates, morphologies and parthenogenetic development after each vitrification step were assessed in comparison with fresh oocytes.
resultsOocytes were retrieved after cryoprotectant agent loading/unloading, and nanoliter droplet encapsulation showed comparable survival rates to fresh oocytes after 24 h in culture. Also, oocytes recovered after vitrification/thawing showed similar morphologies to those of fresh oocytes. Additionally, the rate of oocyte parthenogenetic activation after nanoliter droplet encapsulation was comparable with that observed for fresh oocytes. This nanoliter droplet technology enables the vitrification of oocytes at higher cooling and warming rates using lower cryoprotectant agent levels (i.e., 1.4 M ethylene glycol, 1.1 M dimethyl sulfoxide and 1 M sucrose), thus making it a potential technology to improve oocyte cryopreservation outcomes.
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Registered trials
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