ArticleNucleic acids research2012
Topo IV is the topoisomerase that knots and unknots sister duplexes during DNA replication.
Article in Nucleic acids research, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 52 citations in OpenAlex.
- Quantifying complexity in DNA structures with high resolution Atomic Force Microscopy.Nature communications · 2025Article
- Article
- Electrophoretic Mobility Assay to Separate Supercoiled, Catenated, and Knotted DNA Molecules.Bio-protocol · 2024Article
- Variable DNA topology is an epigenetic generator of physiological heterogeneity in bacterial populations.Molecular microbiology · 2023Review
- Two-Dimensional Gel Electrophoresis to Study the Activity of Type IIA Topoisomerases on Plasmid Replication Intermediates.Biology · 2021Article
- Atomic force microscopy-A tool for structural and translational DNA research.APL bioengineering · 2021Review
- DNA-Topology Simplification by Topoisomerases.Molecules (Basel, Switzerland) · 2021Review
- Closing the DNA replication cycle: from simple circular molecules to supercoiled and knotted DNA catenanes.Nucleic acids research · 2019Review
- DNA supercoiling and transcription in bacteria: a two-way street.BMC molecular and cell biology · 2019Review
- Quantitative disclosure of DNA knot chirality by high-resolution 2D-gel electrophoresis.Nucleic acids research · 2019Article
- Two convergent pathways of DNA knotting in replicating DNA molecules as revealed by θ-curve analysis.Nucleic acids research · 2018Article
- Dynamics of supercoiled DNA with complex knots: large-scale rearrangements and persistent multi-strand interlocking.Nucleic acids research · 2018Article
- DNA knots occur in intracellular chromatin.Nucleic acids research · 2018Article
- The dynamic interplay between DNA topoisomerases and DNA topology.Biophysical reviews · 2016Review
- The Dynamic Interplay Between DNA Topoisomerases and DNA Topology.Biophysical reviews · 2016Article
- How topoisomerase IV can efficiently unknot and decatenate negatively supercoiled DNA molecules without causing their torsional relaxation.Nucleic acids research · 2016Article
- DNA Gyrase of Deinococcus radiodurans is characterized as Type II bacterial topoisomerase and its activity is differentially regulated by PprA in vitro.Extremophiles : life under extreme conditions · 2016Article
- Direct Evidence for the Formation of Precatenanes during DNA Replication.The Journal of biological chemistry · 2015Article
- Electrophoretic mobility of supercoiled, catenated and knotted DNA molecules.Nucleic acids research · 2015Article
- DNA Topoisomerases.EcoSal Plus · 2015Review
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
DNA topology plays a crucial role in all living cells. In prokaryotes, negative supercoiling is required to initiate replication and either negative or positive supercoiling assists decatenation. The role of DNA knots, however, remains a mystery. Knots are very harmful for cells if not removed efficiently, but DNA molecules become knotted in vivo. If knots are deleterious, why then does DNA become knotted? Here, we used classical genetics, high-resolution 2D agarose gel electrophoresis and atomic force microscopy to show that topoisomerase IV (Topo IV), one of the two type-II DNA topoisomerases in bacteria, is responsible for the knotting and unknotting of sister duplexes during DNA replication. We propose that when progression of the replication forks is impaired, sister duplexes become loosely intertwined. Under these conditions, Topo IV inadvertently makes the strand passages that lead to the formation of knots and removes them later on to allow their correct segregation.
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