ArticleAnnals of botany2012
Amplification, contraction and genomic spread of a satellite DNA family (E180) in Medicago (Fabaceae) and allied genera.
Article in Annals of botany, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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13 citing papers in PubMed, 23 citations in OpenAlex.
- From minor loci to major players? Satellite DNA diversification in Crepis sensu stricto.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2025Article
- Oligo-FISH barcode chromosome identification system provides novel insights into the natural chromosome aberrations propensity in the autotetraploid cultivated alfalfa.Horticulture research · 2025Article
- Investigating the Origin and Evolution of PolyploidPlants (Basel, Switzerland) · 2023Article
- Chromosomal view of Lippia alba, a tropical polyploid complex under genome stabilization process.Protoplasma · 2022Article
- Organization and evolution of four differentially amplified tandem repeats in the Cucumis hystrix genome.Planta · 2017Article
- Karyotypic evolution of the Medicago complex: sativa-caerulea-falcata inferred from comparative cytogenetic analysis.BMC evolutionary biology · 2017Article
- Evolutionary dynamics of an at-rich satellite DNA and its contribution to karyotype differentiation in wild diploid Arachis species.Molecular genetics and genomics : MGG · 2017Article
- Evolutionary dynamics of satellite DNA repeats from Phaseolus beans.Protoplasma · 2017Article
- Centromeric and non-centromeric satellite DNA organisation differs in holocentric Rhynchospora species.Chromosoma · 2017Article
- A First Glimpse of Wild Lupin Karyotype Variation As Revealed by Comparative Cytogenetic Mapping.Frontiers in plant science · 2016Article
- Chromosomal structures and repetitive sequences divergence in Cucumis species revealed by comparative cytogenetic mapping.BMC genomics · 2015Article
- Differential amplification of satellite PaB6 in chromosomally hypervariable Prospero autumnale complex (Hyacinthaceae).Annals of botany · 2014Article
- Cloning and characterization of chromosomal markers in alfalfa (Medicago sativa L.).TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2013Article
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Authors and funding
3 authors at 2 institutions in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
BACKGROUND AND
aimsSatellite DNA is a genomic component present in virtually all eukaryotic organisms. The turnover of highly repetitive satellite DNA is an important element in genome organization and evolution in plants. Here we assess the presence and physical distribution of the repetitive DNA E180 family in Medicago and allied genera. Our goals were to gain insight into the karyotype evolution of Medicago using satellite DNA markers, and to evaluate the taxonomic and phylogenetic signal of a satellite DNA family in a genus hypothesized to have a complex evolutionary history.
methodsSeventy accessions from Medicago, Trigonella, Melilotus and Trifolium were analysed by PCR to assess the presence of the repetitive E180 family, and fluorescence in situ hybridization (FISH) was used for physical mapping in somatic chromosomes. KEY
resultsThe E180 repeat unit was PCR-amplified in 37 of 40 taxa in Medicago, eight of 12 species of Trigonella, six of seven species of Melilotus and in two of 11 Trifolium species. Examination of the mitotic chromosomes revealed that only 13 Medicago and two Trigonella species showed FISH signals using the E180 probe. Stronger hybridization signals were observed in subtelomeric and interstitial loci than in the pericentromeric loci, suggesting this satellite family has a preferential genomic location. Not all 13 Medicago species that showed FISH localization of the E180 repeat were phylogenetically related. However, nine of these species belong to the phylogenetically derived clade including the M. sativa and M. arborea complexes.
conclusionsThe use of the E180 family as a phylogenetic marker in Medicago should be viewed with caution. Its amplification appears to have been produced through recurrent and independent evolutionary episodes in both annual and perennial Medicago species as well as in basal and derived clades.
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