ArticleProtist2012
A Trypanosoma brucei protein required for maintenance of the flagellum attachment zone and flagellar pocket ER domains.
Article in Protist, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 27 citations in OpenAlex.
- Whole cell reconstructions of Leishmania mexicana through the cell cycle.PLoS pathogens · 2024Article
- Disruption of Leishmania flagellum attachment zone architecture causes flagellum loss.Molecular microbiology · 2024Article
- Impact of inherent biases built into proteomic techniques: Proximity labeling and affinity capture compared.The Journal of biological chemistry · 2023Article
- Unique Endomembrane Systems and Virulence in Pathogenic Protozoa.Life (Basel, Switzerland) · 2021Review
- To the Surface and Back: Exo- and Endocytic Pathways inFrontiers in cell and developmental biology · 2021Review
- Maintenance of hook complex integrity and centrin arm assembly facilitates flagellum inheritance inThe Journal of biological chemistry · 2020Article
- BOH1 cooperates with Polo-like kinase to regulate flagellum inheritance and cytokinesis initiation inJournal of cell science · 2019Article
- Article
- Cellular landmarks of Trypanosoma brucei and Leishmania mexicana.Molecular and biochemical parasitology · 2019Article
- Identification of TOEFAZ1-interacting proteins reveals key regulators of Trypanosoma brucei cytokinesis.Molecular microbiology · 2018Article
- A Brief View of the Surface Membrane Proteins fromJournal of parasitology research · 2017Review
- The Flagellum Attachment Zone: 'The Cellular Ruler' of Trypanosome Morphology.Trends in parasitology · 2016Review
- Review
- Review
- U-insertion/deletion RNA editing multiprotein complexes and mitochondrial ribosomes in Leishmania tarentolae are located in antipodal nodes adjacent to the kinetoplast DNA.Mitochondrion · 2015Article
- Flagellar membrane proteins in kinetoplastid parasites.IUBMB life · 2015Review
- Assembly and maintenance of the flagellum attachment zone filament in Trypanosoma brucei.Journal of cell science · 2015Article
- Scanning and three-dimensional electron microscopy methods for the study of Trypanosoma brucei and Leishmania mexicana flagella.Methods in cell biology · 2015Article
- New insights into the molecular mechanisms of mitosis and cytokinesis in trypanosomes.International review of cell and molecular biology · 2014Review
- Form and function in the trypanosomal secretory pathway.Current opinion in microbiology · 2012Review
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
Trypanosomes and Leishmanias are important human parasites whose cellular architecture is centred on the single flagellum. In trypanosomes, this flagellum is attached to the cell along a complex flagellum attachment zone (FAZ), comprising flagellar and cytoplasmic components, the integrity of which is required for correct cell morphogenesis and division. The cytoplasmic FAZ cytoskeleton is conspicuously associated with a sheet of endoplasmic reticulum termed the 'FAZ ER'. In the present work, 3D electron tomography of bloodstream form trypanosomes was used to clarify the nature of the FAZ ER. We also identified TbVAP, a T. brucei protein whose knockdown by RNAi in procyclic form cells leads to a dramatic reduction in the FAZ ER, and in the ER associated with the flagellar pocket. TbVAP is an orthologue of VAMP-associated proteins (VAPs), integral ER membrane proteins whose mutation in humans has been linked to familial motor neuron disease. The localisation of tagged TbVAP and the phenotype of TbVAP RNAi in procyclic form trypanosomes are consistent with a function for TbVAP in the maintenance of sub-populations of the ER associated with the FAZ and the flagellar pocket. Nevertheless, depletion of TbVAP did not affect cell viability or cell cycle progression.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.