ArticleVirology journal2011
Rapid detection of newly isolated Tembusu-related Flavivirus by reverse-transcription loop-mediated isothermal amplification assay.
Article in Virology journal, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 47 citations in OpenAlex.
- Comparative Study of DTMUV and LPS on Duck Liver Disease.Veterinary sciences · 2025Article
- Development of a Rapid Visual Detection Assay for Duck Tembusu Virus Using RT-LAMP-CRISPR/Cas12a.Animals : an open access journal from MDPI · 2024Article
- Development of Colorimetric Reverse Transcription Loop-Mediated Isothermal Amplification Assay for Detecting Feline Coronavirus.Animals : an open access journal from MDPI · 2022Article
- Visual Detection of Duck Tembusu Virus With CRISPR/Cas13: A Sensitive and Specific Point-of-Care Detection.Frontiers in cellular and infection microbiology · 2022Article
- Interleukin-2 shows high adjuvanticity for an inactivated vaccine against duck Tembusu virus disease.Poultry science · 2020Article
- Differently Expression Analysis and Function Prediction of Long Non-coding RNAs in Duck Embryo Fibroblast Cells Infected by Duck Tembusu Virus.Frontiers in immunology · 2020Article
- The ubiquitin-proteasome system is necessary for the replication of duck Tembusu virus.Microbial pathogenesis · 2019Article
- Screening and identification of B-cell epitopes within envelope protein of tembusu virus.Virology journal · 2018Article
- Identification of determinants that mediate binding between Tembusu virus and the cellular receptor heat shock protein A9.Journal of veterinary science · 2018Article
- Identification of Glucose-Regulated Protein 78 (GRP78) as a Receptor in BHK-21 Cells for Duck Tembusu Virus Infection.Frontiers in microbiology · 2018Article
- The Emerging Duck Flavivirus Is Not Pathogenic for Primates and Is Highly Sensitive to Mammalian Interferon Antiviral Signaling.Journal of virology · 2016Article
- Rapid detection of the common avian leukosis virus subgroups by real-time loop-mediated isothermal amplification.Virology journal · 2015Article
- Domain I and II from newly emerging goose tembusu virus envelope protein functions as a dominant-negative inhibitor of virus infectivity.Research in veterinary science · 2015Article
- Development of conventional and real-time reverse transcription polymerase chain reaction assays to detect Tembusu virus in Culex tarsalis mosquitoes.The American journal of tropical medicine and hygiene · 2014Article
- Identification of pork in meat products using real-time loop-mediated isothermal amplification.Biotechnology, biotechnological equipment · 2014Article
- Article
- Development of simple and rapid assay to detect viral RNA of tick-borne encephalitis virus by reverse transcription-loop-mediated isothermal amplification.Virology journal · 2013Article
- Structural, antigenic, and evolutionary characterizations of the envelope protein of newly emerging Duck Tembusu Virus.PloS one · 2013Article
- Endpoint visual detection of three genetically modified rice events by loop-mediated isothermal amplification.International journal of molecular sciences · 2012Article
- Sensitive and rapid detection of the new Delhi metallo-beta-lactamase gene by loop-mediated isothermal amplification.Journal of clinical microbiology · 2012Article
Corrections and comments
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Authors and funding
10 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundFrom April 2010 to January 2011, a severe new viral disease had devastated most duck-farming regions in China. This disease affected not only laying ducks but also meat ducks, causing huge economic losses for the poultry industry. The objective of this study is to develop a one-step reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay for the detection of the new virus related to Tembusu-related Flavivirus.
resultsThe RT-LAMP assay is very simple and rapid, and the amplification can be completed within 50 min under isothermal conditions at 63°C by a set of 6 primers targeting the E gene based on the sequences analysis of the newly isolated viruses and other closely related Flavivirus.The monitoring of gene amplification can also be visualized by using SYBR green I fluorescent dye. In addition, the RT-LAMP assay for newly isolated Tembusu-related Flavivirus showed higher sensitivity with an RNA detection-limit of 2 copies/μL compared with 190 copies/μL of the conventional RT-PCR method. The specificity was identified without cross reaction to other common avian pathogens. By screening a panel of clinical samples this method was more feasible in clinical settings and there was higher positive coincidence rate than conventional RT-PCR and virus isolation.
conclusionThe RT-LAMP assay for newly isolated Tembusu-related Flavivirus is a valuable tool for the rapid and real-time detection not only in well-equipped laboratories but also in general conditions.
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