ArticleBMC genomics2011
Correlation of gene expression and protein production rate - a system wide study.
Article in BMC genomics, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed.
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- Impact of GSM-EMW exposure on the markers of oxidative stress in fetal rat liver.Scientific reports · 2023Article
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- Impact of overproduced heterologous protein characteristics on physiological response in Yarrowia lipolytica steady-state-maintained continuous cultures.Applied microbiology and biotechnology · 2020Article
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- Continuous Cultivation as a Tool Toward the Rational Bioprocess Development WithFrontiers in bioengineering and biotechnology · 2020Review
- Secretome Dynamics in a Gram-Positive Bacterial Model.Molecular & cellular proteomics : MCP · 2019Article
- Regulation of plant cell wall degradation by light inFungal biology and biotechnology · 2018Review
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- A novel transcription factor specifically regulates GH11 xylanase genes inBiotechnology for biofuels · 2017Article
- Osmotic conditions could promote scFv antibody production in theBioImpacts : BI · 2017Article
- Abundance of Secreted Proteins ofFrontiers in microbiology · 2017Article
- Article
- A complete annotation of the chromosomes of the cellulase producer Trichoderma reesei provides insights in gene clusters, their expression and reveals genes required for fitness.Biotechnology for biofuels · 2016Article
- Genome wide analysis of protein production load in Trichoderma reesei.Biotechnology for biofuels · 2016Article
- Expression and chromatin structures of cellulolytic enzyme gene regulated by heterochromatin protein 1.Biotechnology for biofuels · 2016Article
- Whole-genome metabolic model ofBiotechnology for biofuels · 2016Article
- Current state of genome-scale modeling in filamentous fungi.Biotechnology letters · 2015Review
Corrections and comments
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Authors and funding
10 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundGrowth rate is a major determinant of intracellular function. However its effects can only be properly dissected with technically demanding chemostat cultivations in which it can be controlled. Recent work on Saccharomyces cerevisiae chemostat cultivations provided the first analysis on genome wide effects of growth rate. In this work we study the filamentous fungus Trichoderma reesei (Hypocrea jecorina) that is an industrial protein production host known for its exceptional protein secretion capability. Interestingly, it exhibits a low growth rate protein production phenotype.
resultsWe have used transcriptomics and proteomics to study the effect of growth rate and cell density on protein production in chemostat cultivations of T. reesei. Use of chemostat allowed control of growth rate and exact estimation of the extracellular specific protein production rate (SPPR). We find that major biosynthetic activities are all negatively correlated with SPPR. We also find that expression of many genes of secreted proteins and secondary metabolism, as well as various lineage specific, mostly unknown genes are positively correlated with SPPR. Finally, we enumerate possible regulators and regulatory mechanisms, arising from the data, for this response.
conclusionsBased on these results it appears that in low growth rate protein production energy is very efficiently used primarly for protein production. Also, we propose that flux through early glycolysis or the TCA cycle is a more fundamental determining factor than growth rate for low growth rate protein production and we propose a novel eukaryotic response to this i.e. the lineage specific response (LSR).
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