ArticleVirology journal2011
Rapid detection of wheat yellow mosaic virus by reverse transcription loop-mediated isothermal amplification.
Article in Virology journal, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 43 citations in OpenAlex.
- One-Step RT-LAMP: A Method for Closed-Tube Colorimetric Detection of RNA Viruses in Plants.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Development of Loop-Mediated Isothermal Amplification (LAMP) Assay for In-Field Detection of American Plum Line Pattern Virus.Viruses · 2024Article
- Rapid and specific detection of wheat spindle streak mosaic virus using RT-LAMP in durum wheat crude leaf extract.PloS one · 2024Article
- Improved protocol forExperimental biology and medicine (Maywood, N.J.) · 2023Article
- Article
- WheatProceedings of the National Academy of Sciences of the United States of America · 2023Article
- Fast and Sensitive Detection of Soil-Borne Cereal Mosaic Virus in Leaf Crude Extract of Durum Wheat.Viruses · 2022Article
- Colorimetric detection platform for banana bunchy top virus (BBTV) based on closed-tube loop mediated isothermal amplification (LAMP) assay.Virusdisease · 2022Article
- Loop-Mediated Isothermal Amplification for Detection of Plant Pathogens in Wheat (Frontiers in plant science · 2022Review
- Identification of a Novel Quinvirus in the FamilyFrontiers in microbiology · 2021Article
- Loop Mediated Isothermal Amplification: Principles and Applications in Plant Virology.Plants (Basel, Switzerland) · 2020Review
- Variable 3'polyadenylation of Wheat yellow mosaic virus and its novel effects on translation and replication.Virology journal · 2019Article
- Detection of murine norovirus by reverse transcription loop-mediated isothermal amplification.Journal of virological methods · 2014Article
- A one step real-time RT-PCR assay for the quantitation of Wheat yellow mosaic virus (WYMV).Virology journal · 2013Article
- Pepino mosaic virus genotype shift in North America and development of a loop-mediated isothermal amplification for rapid genotype identification.Virology journal · 2013Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
For the detection of wheat yellow mosaic virus (WYMV), we established a reverse transcription loop-mediated isothermal amplification (RT-LAMP) method. Using Primer Explorer software, four sets of primers were designed and RT-LAMP assay reaction conditions were optimized. The RT-LAMP was performed at different times by four primer sets. Agarose gel analysis showed that WYMV could be detected after 30 min with the primer set III and after 45 min with the other three primer sets, both under the 80-min reaction time. RT-LAMP had the same results with the four primer sets, thus primer set III and 65°C for 80 min reaction were selected for virus detection. There was no significant different when avian myeloblastosis virus (AMV) and moloney murine leukemia virus (M-MLV) RT-LAMP with the four primer sets and M-MLV was chosen due to its relatively cheap price. The result on specificity showed that the assay could amplify WYMV specifically, and the sensitivity comparison showed that the RT-LAMP was 100 times more sensitive than conventional reverse-transcriptase-polymerase chain reaction (RT-PCR). Overall, RT-LAMP was found to be a simple, specific, sensitive, convenient and time-saving method for WYMV detection.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.