ArticlePlant physiology2012
Co-overexpression FIT with AtbHLH38 or AtbHLH39 in Arabidopsis-enhanced cadmium tolerance via increased cadmium sequestration in roots and improved iron homeostasis of shoots.
Article in Plant physiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 86 papers.
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Who cites it
86 citing papers in PubMed, 241 citations in OpenAlex.
- Transcription Factor TCP9 EnhancesPlants (Basel, Switzerland) · 2026Article
- Plastid G6PD3 and cytosolic G6PD5/6 are involved in the Ustilago sp. HFJ311-enhanced tolerance to cadmium stress in Arabidopsis.BMC plant biology · 2026Article
- Advances in Molecular Mechanisms Underlying Cadmium Accumulation and Detoxification in Durum Wheat (International journal of molecular sciences · 2026Review
- Lobularia maritima essential oil modulates heavy-metal transporter expression and mitigates cadmium stress in durum wheat.Protoplasma · 2026Article
- Iron status modulates cadmium tolerance: mechanistic insights fromFrontiers in plant science · 2026Article
- Genome-wide identification and expression analysis of salt-responsive bHLH transcription factors in the wheat (Frontiers in plant science · 2026Article
- Effects of foliar applied asparagine, glycine and citric acid on cadmium uptake and yield in wheat.PeerJ · 2025Article
- Comparative Physiological and Transcriptomics Profiling Provides Integrated Insight into Melatonin Mediated Salt and Copper Stress Tolerance inPlants (Basel, Switzerland) · 2024Article
- Leveraging multi-omics tools to comprehend responses and tolerance mechanisms of heavy metals in crop plants.Functional & integrative genomics · 2024Review
- GhRCD1 promotes cotton tolerance to cadmium by regulating the GhbHLH12-GhMYB44-GhHMA1 transcriptional cascade.Plant biotechnology journal · 2024Article
- The Uptake, Transfer, and Detoxification of Cadmium in Plants and Its Exogenous Effects.Cells · 2024Review
- AlfalfaFrontiers in plant science · 2024Article
- Transcription factors involved in plant responses to cadmium-induced oxidative stress.Frontiers in plant science · 2024Review
- Genome-wide identification ofPeerJ · 2024Article
- Article
- BRUTUS-LIKE (BTSL) E3 ligase-mediated fine-tuning of Fe regulation negatively affects Zn tolerance of Arabidopsis.Journal of experimental botany · 2023Article
- The TabHLH094-TaMYC8 complex mediates the cadmium response in wheat.Molecular breeding : new strategies in plant improvement · 2023Article
- The TabHLH35-TaWAK20-TaSPL5 pathway positively regulates Cd stress in wheat.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2023Article
- Characterization and Expression Analysis of Four Cadmium-Tolerance-Associated Genes ofBiology · 2023Article
- Multilayered regulation of iron homeostasis in Arabidopsis.Frontiers in plant science · 2023Review
26 more citing papers are in PubMed but not listed here.
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Authors and funding
12 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cadmium (Cd) is toxic to plant cells. Under Cd exposure, the plant displayed leaf chlorosis, which is a typical symptom of iron (Fe) deficiency. Interactions of Cd with Fe have been reported. However, the molecular mechanisms of Cd-Fe interactions are not well understood. Here, we showed that FER-like Deficiency Induced Transcripition Factor (FIT), AtbHLH38, and AtbHLH39, three basic helix-loop-helix transcription factors involved in Fe homeostasis in plants, also play important roles in Cd tolerance. The gene expression analysis showed that the expression of FIT, AtbHLH38, and AtbHLH39 was up-regulated in the roots of plants treated with Cd. The plants overexpressing AtbHLH39 and double-overexpressing FIT/AtbHLH38 and FIT/AtbHLH39 exhibited more tolerance to Cd exposure than wild type, whereas no Cd tolerance was observed in plants overexpressing either AtbHLH38 or FIT. Further analysis revealed that co-overexpression of FIT with AtbHLH38 or AtbHLH39 constitutively activated the expression of Heavy Metal Associated3 (HMA3), Metal Tolerance Protein3 (MTP3), Iron Regulated Transporter2 (IRT2), and Iron Regulated Gene2 (IREG2), which are involved in the heavy metal detoxification in Arabidopsis (Arabidopis thaliana). Moreover, co-overexpression of FIT with AtbHLH38 or AtbHLH39 also enhanced the expression of NICOTIANAMINE SYNTHETASE1 (NAS1) and NAS2, resulting in the accumulation of nicotiananamine, a crucial chelator for Fe transportation and homeostasis. Finally, we showed that maintaining high Fe content in shoots under Cd exposure could alleviate the Cd toxicity. Our results provide new insight to understand the molecular mechanisms of Cd tolerance in plants.
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