ArticleProceedings of the National Academy of Sciences of the United States of America2012
Centromere-tethered Mps1 pombe homolog (Mph1) kinase is a sufficient marker for recruitment of the spindle checkpoint protein Bub1, but not Mad1.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 35 citations in OpenAlex.
- Cell-cycle phospho-regulation of the kinetochore.Current genetics · 2021Review
- Polo kinase recruitment via the constitutive centromere-associated network at the kinetochore elevates centromeric RNA.PLoS genetics · 2020Article
- Aurora B prevents premature removal of spindle assembly checkpoint proteins from the kinetochore: A key role for Aurora B in mitosis.Oncotarget · 2018Article
- Rad52 phosphorylation by Ipl1 and Mps1 contributes to Mps1 kinetochore localization and spindle assembly checkpoint regulation.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Purification and characterisation of the fission yeast Ndc80 complex.Protein expression and purification · 2017Article
- Generation of a Spindle Checkpoint Arrest from Synthetic Signaling Assemblies.Current biology : CB · 2017Article
- Inositol Pyrophosphate Kinase Asp1 Modulates Chromosome Segregation Fidelity and Spindle Function in Schizosaccharomyces pombe.Molecular and cellular biology · 2016Article
- Synthetic Physical Interactions Map Kinetochore-Checkpoint Activation Regions.G3 (Bethesda, Md.) · 2016Article
- The hairpin region of Ndc80 is important for the kinetochore recruitment of Mph1/MPS1 in fission yeast.Cell cycle (Georgetown, Tex.) · 2016Article
- The kinetochore encodes a mechanical switch to disrupt spindle assembly checkpoint signalling.Nature cell biology · 2015Article
- Signalling dynamics in the spindle checkpoint response.Nature reviews. Molecular cell biology · 2014Review
- Mad1 kinetochore recruitment by Mps1-mediated phosphorylation of Bub1 signals the spindle checkpoint.Genes & development · 2014Article
- Bub3 reads phosphorylated MELT repeats to promote spindle assembly checkpoint signaling.eLife · 2013Article
- Drosophila Polo regulates the spindle assembly checkpoint through Mps1-dependent BubR1 phosphorylation.The EMBO journal · 2013Article
- Making an effective switch at the kinetochore by phosphorylation and dephosphorylation.Chromosoma · 2013Review
- An E2 enzyme Ubc11 is required for ubiquitination of Slp1/Cdc20 and spindle checkpoint silencing in fission yeast.Cell cycle (Georgetown, Tex.) · 2013Article
- The final link: tapping the power of chemical genetics to connect the molecular and biologic functions of mitotic protein kinases.Molecules (Basel, Switzerland) · 2012Review
- MPS1/Mph1 phosphorylates the kinetochore protein KNL1/Spc7 to recruit SAC components.Nature cell biology · 2012Article
- Spindle checkpoint-independent inhibition of mitotic chromosome segregation by Drosophila Mps1.Molecular biology of the cell · 2012Article
- Phosphoregulation of Spc105 by Mps1 and PP1 regulates Bub1 localization to kinetochores.Current biology : CB · 2012Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The spindle checkpoint delays the onset of anaphase until all of the chromosomes properly achieve bipolar attachment to the spindle. It has been shown that unattached kinetochores are the site that emits a signal for activation of the checkpoint. Although the components of the checkpoint such as Bub1, Mad1 and Mad2 selectively accumulate at unattached kinetochores, the answer to how they recognize unattached kinetochores has remained elusive. Mps1 pombe homolog (Mph1) kinase has been shown to function upstream of most of the components of the checkpoint and thus it is thought to recognize unattached kinetochores by itself and recruit other components. In this study we have expressed a fusion protein of Mph1 and Ndc80 (a kinetochore protein of the outer plate) and shown that the fusion protein arrests cell cycle progression in a spindle-checkpoint\x{2013}dependent manner in fission yeast. When expression of Mad2 is turned off, the cells grow normally with Mph1 constitutively localized at centromeres/kinetochores. Under this condition, Bub1 can be found with Mph1 throughout the cell cycle, indicating that localization of Mph1 at centromeres/kinetochores is sufficient to recruit Bub1. In contrast, Mad1 is found to transiently localize at kinetochores, which are presumably unattached to the spindle, but soon it dissociates from kinetochores. We propose that Mph1 is a sufficient marker for recruitment of Bub1. Mad1, in contrast, requires an additional condition/component for stable association with kinetochores.
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