ArticleProceedings of the National Academy of Sciences of the United States of America2012
Tripartite organization of centromeric chromatin in budding yeast.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 72 papers.
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Who cites it
72 citing papers in PubMed, 113 citations in OpenAlex.
- Towards a unified model of aneuploid karyotype dynamics.PLoS genetics · 2026Article
- Direct observation of interdependent and hierarchical kinetochore assembly on individual centromeres.Nucleic acids research · 2025Article
- High-resolution analysis of human centromeric chromatin.Life science alliance · 2025Article
- Cryo-EM Analysis of a Unique Subnucleosome Containing Centromere-Specific Histone Variant CENP-A.Genes to cells : devoted to molecular & cellular mechanisms · 2025Article
- The yeast genome is globally accessible in living cells.Nature structural & molecular biology · 2025Article
- DNA satellite and chromatin organization at mouse centromeres and pericentromeres.Genome biology · 2024Article
- ISW1a modulates cohesin distribution in centromeric and pericentromeric regions.Nucleic acids research · 2023Article
- Cryo-EM structure of the complete inner kinetochore of the budding yeast point centromere.Science advances · 2023Article
- Nuclear genome organization in fungi: from gene folding to Rabl chromosomes.FEMS microbiology reviews · 2023Article
- Nanoscale structural organization and stoichiometry of the budding yeast kinetochore.The Journal of cell biology · 2023Article
- Cell cycle control of kinetochore assembly.Nucleus (Austin, Tex.) · 2022Review
- Structure of the human inner kinetochore CCAN complex and its significance for human centromere organization.Molecular cell · 2022Article
- Molecular Complexes at Euchromatin, Heterochromatin and Centromeric Chromatin.International journal of molecular sciences · 2021Review
- Cooperative binding between distant transcription factors is a hallmark of active enhancers.Molecular cell · 2021Article
- Structural and dynamic mechanisms of CBF3-guided centromeric nucleosome formation.Nature communications · 2021Article
- Repetitive Elements Contribute to the Diversity and Evolution of Centromeres in the Fungal GenusmBio · 2020Article
- Centromeres under Pressure: Evolutionary Innovation in Conflict with Conserved Function.Genes · 2020Review
- Article
- Architecture of the CBF3-centromere complex of the budding yeast kinetochore.Nature structural & molecular biology · 2018Article
- A unified computational framework for modeling genome-wide nucleosome landscape.Physical biology · 2018Article
12 more citing papers are in PubMed but not listed here.
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
The centromere is the genetic locus that organizes the proteinaceous kinetochore and is responsible for attachment of the chromosome to the spindle at mitosis and meiosis. In most eukaryotes, the centromere consists of highly repetitive DNA sequences that are occupied by nucleosomes containing the CenH3 histone variant, whereas in budding yeast, a ∼120-bp centromere DNA element (CDE) that is sufficient for centromere function is occupied by a single right-handed histone variant CenH3 (Cse4) nucleosome. However, these in vivo observations are inconsistent with in vitro evidence for left-handed octameric CenH3 nucleosomes. To help resolve these inconsistencies, we characterized yeast centromeric chromatin at single base-pair resolution. Intact particles containing both Cse4 and H2A are precisely protected from micrococcal nuclease over the entire CDE of all 16 yeast centromeres in both solubilized chromatin and the insoluble kinetochore. Small DNA-binding proteins protect CDEI and CDEIII and delimit the centromeric nucleosome to the ∼80-bp CDEII, only enough for a single DNA wrap. As expected for a tripartite organization of centromeric chromatin, loss of Cbf1 protein, which binds to CDEI, both reduces the size of the centromere-protected region and shifts its location toward CDEIII. Surprisingly, Cse4 overproduction caused genome-wide misincorporation of nonfunctional CenH3-containing nucleosomes that protect ∼135 base pairs and are preferentially enriched at sites of high nucleosome turnover. Our detection of two forms of CenH3 nucleosomes in the yeast genome, a singly wrapped particle at the functional centromere and octamer-sized particles on chromosome arms, reconcile seemingly conflicting in vivo and in vitro observations.
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