ArticleMethods in molecular biology (Clifton, N.J.)2012
Monitoring dynamic binding of chromatin proteins in vivo by fluorescence recovery after photobleaching.
Article in Methods in molecular biology (Clifton, N.J.), 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
17 citing papers in PubMed.
- Article
- Short activation domains control chromatin association of transcription factors.bioRxiv : the preprint server for biology · 2025Article
- Mitotic bookmarking redundancy by nuclear receptors in pluripotent cells.Nature structural & molecular biology · 2024Article
- An activating mutation of the NSD2 histone methyltransferase drives oncogenic reprogramming in acute lymphocytic leukemia.Oncogene · 2019Article
- The SET1 Complex Selects Actively Transcribed Target Genes via Multivalent Interaction with CpG Island Chromatin.Cell reports · 2017Article
- PCGF3/5-PRC1 initiates Polycomb recruitment in X chromosome inactivation.Science (New York, N.Y.) · 2017Article
- Mitotic binding of Esrrb marks key regulatory regions of the pluripotency network.Nature cell biology · 2016Article
- On the way of revealing coactivator complexes cross-talk during transcriptional activation.Cell & bioscience · 2016Review
- Chromatin structure and gene regulation: a dynamic view of enhancer function.Nucleus (Austin, Tex.) · 2015Article
- Nup50 is required for cell differentiation and exhibits transcription-dependent dynamics.Molecular biology of the cell · 2014Article
- Fluorescent probes for exploring plant cell wall deconstruction: a review.Molecules (Basel, Switzerland) · 2014Review
- Article
- Intracellular dynamics of archaeal FANCM homologue Hef in response to halted DNA replication.Nucleic acids research · 2013Article
- Quantitative analysis of self-association and mobility of annexin A4 at the plasma membrane.Biophysical journal · 2013Article
- Quantifying the transcriptional output of single alleles in single living mammalian cells.Nature protocols · 2013Article
- Complex dynamics of transcription regulation.Biochimica et biophysica acta · 2012Review
- Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
Fluorescence recovery after photobleaching (FRAP) has now become widely used to investigate nuclear protein binding to chromatin in live cells. FRAP can be applied qualitatively to assess if chromatin binding interactions are altered by various biological perturbations. It can also be applied semi-quantitatively to allow numerical comparisons between FRAP curves, and even fully quantitatively to yield estimates of in vivo diffusion constants and nuclear protein binding rates to chromatin. Here we describe how FRAP data should be collected and processed for these qualitative, semi-quantitative, and quantitative analyses.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.