ArticleBMC microbiology2011
Cloning and expression of a novel lactococcal aggregation factor from Lactococcus lactis subsp. lactis BGKP1.
Article in BMC microbiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 41 citations in OpenAlex.
- Toward safer and sustainable food preservation: a comprehensive review of bacteriocins in the food industry.Bioscience reports · 2025Review
- Identification of an operon and its regulator required for autoaggregation inApplied and environmental microbiology · 2023Article
- Polymer Adhesin Domains in Gram-Positive Cell Surface Proteins.Frontiers in microbiology · 2020Review
- Binding activity to intestinal cells and transient colonization in mice of two Lactobacillus paracasei subsp. paracasei strains with high aggregation potential.World journal of microbiology & biotechnology · 2019Article
- TheFrontiers in microbiology · 2019Article
- Functional Characterization of the Lactolisterin BU Gene Cluster ofFrontiers in microbiology · 2018Article
- Article
- Surface Proteins ofFrontiers in microbiology · 2017Review
- Novel Aggregation Promoting Factor AggE Contributes to the Probiotic Properties ofFrontiers in microbiology · 2017Article
- LsbB Bacteriocin Interacts with the Third Transmembrane Domain of the YvjB Receptor.Applied and environmental microbiology · 2016Article
- Article
- Shortening of the Lactobacillus paracasei subsp. paracasei BGNJ1-64 AggLb Protein Switches Its Activity from Auto-aggregation to Biofilm Formation.Frontiers in microbiology · 2016Article
- Technological properties and probiotic potential of Lactobacillus fermentum strains isolated from West African fermented millet dough.BMC microbiology · 2015Article
- Plasmids from Food Lactic Acid Bacteria: Diversity, Similarity, and New Developments.International journal of molecular sciences · 2015Review
- Isolation and Characterisation of Bacteriocin and Aggregation-Promoting Factor Production inFood technology and biotechnology · 2015Article
- Impact of the exopolysaccharide layer on biofilms, adhesion and resistance to stress in Lactobacillus johnsonii FI9785.BMC microbiology · 2015Article
- Exopolysaccharide production and ropy phenotype are determined by two gene clusters in putative probiotic strain Lactobacillus paraplantarum BGCG11.Applied and environmental microbiology · 2015Article
- Article
- Proteinase PrtP impairs lactococcin LcnB activity in Lactococcus lactis BGMN1-501: new insights into bacteriocin regulation.Frontiers in microbiology · 2015Article
- Aggregation factor as an inhibitor of bacterial binding to gut mucosa.Microbial ecology · 2014Article
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundAggregation may play a main role in the adhesion of bacteria to the gastrointestinal epithelium and their colonization ability, as well as in probiotic effects through co-aggregation with intestinal pathogens and their subsequent removal. The aggregation phenomenon in lactococci is directly associated with the sex factor and lactose plasmid co-integration event or duplication of the cell wall spanning (CWS) domain of PrtP proteinase.
resultsLactococcus lactis subsp. lactis BGKP1 was isolated from artisanal semi-hard homemade cheese and selected due to its strong auto-aggregation phenotype. Subsequently, non-aggregating derivative (Agg-) of BGKP1, designated as BGKP1-20, was isolated, too. Comparative analysis of cell surface proteins of BGKP1 and derivative BGKP1-20 revealed a protein of approximately 200 kDa only in the parental strain BGKP1. The gene involved in aggregation (aggL) was mapped on plasmid pKP1 (16.2 kb), cloned and expressed in homologous and heterologous lactococci and enterococci. This novel lactococcal aggregation protein was shown to be sufficient for cell aggregation in all tested hosts. In addition to the aggL gene, six more ORFs involved in replication (repB and repX), restriction and modification (hsdS), transposition (tnp) and possible interaction with mucin (mbpL) were also located on plasmid pKP1.
conclusionAggL is a new protein belonging to the collagen-binding superfamily of proteins and is sufficient for cell aggregation in lactococci.
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