ArticlePLoS neglected tropical diseases2011
In vivo expression of Salmonella enterica serotype Typhi genes in the blood of patients with typhoid fever in Bangladesh.
Article in PLoS neglected tropical diseases, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed.
- CRISPR-Cas system positively regulates virulence of Salmonella enterica serovar Typhimurium.Gut pathogens · 2024Article
- Characterization of the multidrug efflux transporter styMdtM from Salmonella enterica serovar Typhi.Proteins · 2021Article
- Survival of Salmonella Under Heat Stress is Associated with the Presence/Absence of CRISPR Cas Genes and Iron Levels.Current microbiology · 2021Article
- Enteric Fever Diagnosis: Current Challenges and Future Directions.Pathogens (Basel, Switzerland) · 2021Review
- New Roles for Two-Component System Response Regulators ofMicroorganisms · 2020Article
- Mimicking the human environment in mice reveals that inhibiting biotin biosynthesis is effective against antibiotic-resistant pathogens.Nature microbiology · 2020Article
- Characterization of CRISPR-Cas systems in the Ralstonia solanacearum species complex.Molecular plant pathology · 2019Article
- Virulotyping of Salmonella enterica serovar Typhi isolates from Pakistan: Absence of complete SPI-10 in Vi negative isolates.PLoS neglected tropical diseases · 2018Article
- Pathogenomics of Virulence Traits ofFrontiers in microbiology · 2018Article
- A Comprehensive Assessment of the Genetic Determinants in Salmonella Typhimurium for Resistance to Hydrogen Peroxide Using Proteogenomics.Scientific reports · 2017Article
- An evaluation of purified Salmonella Typhi protein antigens for the serological diagnosis of acute typhoid fever.The Journal of infection · 2017Article
- Transcriptomics in Human Challenge Models.Frontiers in immunology · 2017Review
- A magneto-DNA nanoparticle system for the rapid and sensitive diagnosis of enteric fever.Scientific reports · 2016Article
- The Bacterial iprA Gene Is Conserved across Enterobacteriaceae, Is Involved in Oxidative Stress Resistance, and Influences Gene Expression in Salmonella enterica Serovar Typhimurium.Journal of bacteriology · 2016Article
- Fluorescence Polarization Based Nucleic Acid Testing for Rapid and Cost-Effective Diagnosis of Infectious Disease.Chemistry (Weinheim an der Bergstrasse, Germany) · 2015Article
- Epidemiology, Clinical Presentation, Laboratory Diagnosis, Antimicrobial Resistance, and Antimicrobial Management of Invasive Salmonella Infections.Clinical microbiology reviews · 2015Review
- Rapidly Escalating Hepcidin and Associated Serum Iron Starvation Are Features of the Acute Response to Typhoid Infection in Humans.PLoS neglected tropical diseases · 2015Article
- Host hydrogen rather than that produced by the pathogen is important for Salmonella enterica serovar Typhimurium virulence.Infection and immunity · 2015Article
- Immunoproteomic analysis of antibody in lymphocyte supernatant in patients with typhoid fever in Bangladesh.Clinical and vaccine immunology : CVI · 2014Article
- The role of CRISPR-Cas systems in virulence of pathogenic bacteria.Microbiology and molecular biology reviews : MMBR · 2014Review
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22 authors.
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Abstract
backgroundSalmonella enterica serotype Typhi is the cause of typhoid fever. It is a human-restricted pathogen, and few data exist on S. Typhi gene expression in humans. METHODOLOGY/PRINCIPAL
findingsWe applied an RNA capture and amplification technique, Selective Capture of Transcribed Sequences (SCOTS), and microarray hybridization to identify S. Typhi transcripts expressed in the blood of five humans infected with S. Typhi in Bangladesh. In total, we detected the expression of mRNAs for 2,046 S. Typhi genes (44% of the S. Typhi genome) in human blood; expression of 912 genes was detected in all 5 patients, and expression of 1,100 genes was detected in 4 or more patients. Identified transcripts were associated with the virulence-associated PhoP regulon, Salmonella pathogenicity islands, the use of alternative carbon and energy sources, synthesis and transport of iron, thiamine, and biotin, and resistance to antimicrobial peptides and oxidative stress. The most highly represented group were genes currently annotated as encoding proteins designated as hypothetical, unknown, or unclassified. Of the 2,046 detected transcripts, 1,320 (29% of the S. Typhi genome) had significantly different levels of detection in human blood compared to in vitro cultures; detection of 141 transcripts was significantly different in all 5 patients, and detection of 331 transcripts varied in at least 4 patients. These mRNAs encode proteins of unknown function, those involved in energy metabolism, transport and binding, cell envelope, cellular processes, and pathogenesis. We confirmed increased expression of a subset of identified mRNAs by quantitative-PCR. CONCLUSIONS/SIGNIFICANCE: We report the first characterization of bacterial transcriptional profiles in the blood of patients with typhoid fever. S. Typhi is an important global pathogen whose restricted host range has greatly inhibited laboratory studies. Our results suggest that S. Typhi uses a largely uncharacterized genetic repertoire to survive within cells and utilize alternate energy sources during infection.
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