Trial reportThe Journal of biological chemistry2012
Protein modification by deamidation indicates variations in joint extracellular matrix turnover.
Trial report in The Journal of biological chemistry, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
24 citing papers in PubMed, 1 synthesis or guideline pooled it, 53 citations in OpenAlex.
- What is the utility of biomarkers for assessing the pathophysiology of hip osteoarthritis? A systematic review.Clinical orthopaedics and related research · 2015Pooled it
- Serum and Clinical Factors Associated with Total Knee Arthroplasty in Patients with Knee Osteoarthritis.Life (Basel, Switzerland) · 2026Article
- Biomarkers of aging as it relates osteoarthritis: we can't improve what we can't measure.Connective tissue research · 2025Review
- Thick or Thin? Implications of Cartilage Architecture for Osteoarthritis Risk in Sedentary Lifestyles.Biomedicines · 2025Review
- UBC9-Mediated SUMO Pathway Drives Prohibitin-1 Nuclear Accumulation and PITX1 Repression in Primary Osteoarthritis.International journal of molecular sciences · 2025Article
- Comprehensive characterization of pathogenic synovial fluid extracellular vesicles from knee osteoarthritis.Clinical immunology (Orlando, Fla.) · 2023Article
- Metabolic labeling of secreted matrix to investigate cell-material interactions in tissue engineering and mechanobiology.Nature protocols · 2022Review
- An In Vivo Stable Isotope Labeling Method to Investigate Individual Matrix Protein Synthesis, Ribosomal Biogenesis, and Cellular Proliferation in Murine Articular Cartilage.Function (Oxford, England) · 2022Article
- Chondrocytic and pharmacokinetic properties of Phlpp inhibitors.Osteoarthritis and cartilage open · 2021Article
- Soluble biological markers in osteoarthritis.Therapeutic advances in musculoskeletal disease · 2021Review
- Biomarkers of Joint Damage in Osteoarthritis: Current Status and Future Directions.Mediators of inflammation · 2021Review
- Analysis of "old" proteins unmasks dynamic gradient of cartilage turnover in human limbs.Science advances · 2019Article
- Phlpp inhibitors block pain and cartilage degradation associated with osteoarthritis.Journal of orthopaedic research : official publication of the Orthopaedic Research Society · 2018Article
- Meta-analysis of urinary C-terminal telopeptide of type II collagen as a biomarker in osteoarthritis diagnosis.Journal of orthopaedic translation · 2018Article
- Biomarkers reflect differences in osteoarthritis phenotypes of the lumbar spine: the Johnston County Osteoarthritis Project.Osteoarthritis and cartilage · 2017Article
- Cartilage biomarkers in the osteoarthropathy of alkaptonuria reveal low turnover and accelerated ageing.Rheumatology (Oxford, England) · 2017Article
- Aspartic acid racemization reveals a high turnover state in knee compared with hip osteoarthritic cartilage.Osteoarthritis and cartilage · 2016Article
- Call for standardized definitions of osteoarthritis and risk stratification for clinical trials and clinical use.Osteoarthritis and cartilage · 2015Review
- OARSI Clinical Trials Recommendations: Soluble biomarker assessments in clinical trials in osteoarthritis.Osteoarthritis and cartilage · 2015Review
- The Natural History of Femoroacetabular Impingement.Frontiers in surgery · 2015Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors at 3 institutions in 1 country.
Funding
Abstract
As extracellular proteins age, they undergo and accumulate nonenzymatic post-translational modifications that cannot be repaired. We hypothesized that these could be used to systemically monitor loss of extracellular matrix due to chronic arthritic diseases such as osteoarthritis (OA). To test this, we predicted sites of deamidation in cartilage oligomeric matrix protein (COMP) and confirmed, by mass spectroscopy, the presence of deamidated (Asp(64)) and native (Asn(64)) COMP epitopes (mean 0.95% deamidated COMP (D-COMP) relative to native COMP) in cartilage. An Asp(64), D-COMP-specific ELISA was developed using a newly created monoclonal antibody 6-1A12. In a joint replacement study, serum D-COMP (p = 0.017), but not total COMP (p = 0.5), declined significantly after replacement demonstrating a joint tissue source for D-COMP. In analyses of 450 participants from the Johnston County Osteoarthritis Project controlled for age, gender, and race, D-COMP was associated with radiographic hip (p < 0.0001) but not knee (p = 0.95) OA severity. In contrast, total COMP was associated with radiographic knee (p < 0.0001) but not hip (p = 0.47) OA severity. D-COMP was higher in soluble proteins extracted from hip cartilage proximal to OA lesions compared with remote from lesions (p = 0.007) or lesional and remote OA knee (p < 0.01) cartilage. Total COMP in cartilage did not vary by joint site or proximity to the lesion. This study demonstrates the presence of D-COMP in articular cartilage and the systemic circulation, and to our knowledge, it is the first biomarker to show specificity for a particular joint site. We believe that enrichment of deamidated epitope in hip OA cartilage indicates a lesser repair response of hip OA compared with knee OA cartilage.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.