ArticleNature methods2011
Principles for applying optogenetic tools derived from direct comparative analysis of microbial opsins.
Article in Nature methods, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 410 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
410 citing papers in PubMed, 793 citations in OpenAlex.
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- Parallel cholinergic circuit in oculomotor nucleus to control eye movements and REM sleep.Nature communications · 2026Article
- Theoretical analysis of low power synergistic sono-optogenetic control of calcium-dependent synaptic plasticity.Scientific reports · 2026Article
- Multi-site temporal control of optogenetic stimulation enhances firing frequencies in peripheral nerves.bioRxiv : the preprint server for biology · 2026Article
- Val109 and Ile170 contribute to channel function in Guillardia theta-derived anion channelrhodopsins.Scientific reports · 2026Article
- Hemodynamic and Electrophysiological Progression of the Rose Bengal Photothrombotic Stroke Model in Mice.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2026Article
- Desensitization of opsin responses during all-optical interrogation depends on imaging parameters.Neurophotonics · 2026Article
- Dynamic nanoscale architecture of synaptic vesicle fusion in mouse hippocampal neurons.Nature communications · 2025Article
- Metabolic control of porin permeability influences antibiotic resistance in Escherichia coli.Nature microbiology · 2025Article
- Roadmap for direct and indirect translation of optogenetics into discoveries and therapies for humans.Nature neuroscience · 2025Review
- Implantable Light-Powered Human Designer Cells for Electrical Energy Generation.Advanced materials (Deerfield Beach, Fla.) · 2025Article
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- XPRESSO: Rapid genetic engineering of human pluripotent stem cells for durable overexpression using a modular anti-silencing vector.Stem cell reports · 2025Article
- Flexible modeling of large-scale neural network stimulation: Electrical and optical extensions to The Virtual Electrode Recording Tool for EXtracellular Potentials (VERTEX).Journal of neuroscience methods · 2025Article
- Characterizing optogenetically mediated rebound effects in anaesthetized mouse primary visual cortex.The Journal of physiology · 2025Article
350 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
12 authors at 3 institutions in 2 countries.
Funding
Abstract
Diverse optogenetic tools have allowed versatile control over neural activity. Many depolarizing and hyperpolarizing tools have now been developed in multiple laboratories and tested across different preparations, presenting opportunities but also making it difficult to draw direct comparisons. This challenge has been compounded by the dependence of performance on parameters such as vector, promoter, expression time, illumination, cell type and many other variables. As a result, it has become increasingly complicated for end users to select the optimal reagents for their experimental needs. For a rapidly growing field, critical figures of merit should be formalized both to establish a framework for further development and so that end users can readily understand how these standardized parameters translate into performance. Here we systematically compared microbial opsins under matched experimental conditions to extract essential principles and identify key parameters for the conduct, design and interpretation of experiments involving optogenetic techniques.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.