ArticleJournal of industrial microbiology & biotechnology2012
Gene cloning, expression, and biochemical characterization of an alkali-tolerant β-mannanase from Humicola insolens Y1.
Article in Journal of industrial microbiology & biotechnology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- Alkaliphilic/Alkali-Tolerant Fungi: Molecular, Biochemical, and Biotechnological Aspects.Journal of fungi (Basel, Switzerland) · 2023Review
- Applications of Microbial β-Mannanases.Frontiers in bioengineering and biotechnology · 2020Review
- Galactomannan degradation by thermophilic enzymes: a hot topic for biotechnological applications.World journal of microbiology & biotechnology · 2019Review
- Characterization of a novel low-temperature-active, alkaline and sucrose-tolerant invertase.Scientific reports · 2016Article
- The use of T-DNA insertional mutagenesis to improve cellulase production by the thermophilic fungus Humicola insolens Y1.Scientific reports · 2016Article
- A novel surfactant-, NaCl-, and protease-tolerant β-mannanase from Bacillus sp. HJ14.Folia microbiologica · 2016Article
- Article
- Molecular and biochemical characterizations of a new low-temperature active mannanase.Folia microbiologica · 2015Article
- A Neutral Thermostable β-1,4-Glucanase from Humicola insolens Y1 with Potential for Applications in Various Industries.PloS one · 2015Article
- Molecular characterization of a new alkaline-tolerant xylanase from Humicola insolens Y1.BioMed research international · 2015Article
- Truncation of a mannanase from Trichoderma harzianum improves its enzymatic properties and expression efficiency in Trichoderma reesei.Journal of industrial microbiology & biotechnology · 2014Article
- Comparison of expression systems for the extracellular production of mannanase Man23 originated from Bacillus subtilis B23.Microbial cell factories · 2013Article
- Microbial enzymes with special characteristics for biotechnological applications.Biomolecules · 2013Article
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Authors and funding
6 authors.
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Abstract
In this article, we firstly report a highly alkali-tolerant fungal β-mannanase from Humicola insolens Y1. The full-length cDNA of the β-mannanase, designated as man5A, has an open reading frame of 1,233 bp that encodes a 411-amino acid polypeptide (Man5A) with a calculated molecular mass of 42.3 kDa. The deduced sequence of Man5A comprises a putative 20-residue signal peptide and a catalytic domain belonging to glycoside hydrolase family 5, and displays 61-85% identities with hypothetical proteins and 32-39% with experimentally verified fungal β-mannanases. Purified recombinant Man5A produced by Pichia pastoris has a specific activity of 1,122 U mg⁻¹ and exhibits optimal activity at pH 5.5 and 70°C. Distinct from other reported fungal β-mannanases, Man5A is highly alkali tolerant, exhibiting 45 and 36% of the maximal activity at pH 8.0 and 9.0, respectively, and more than 10% activity even at pH 10.0. Moreover, Man5A has excellent pH stability at pH 5.0-12.0 and is highly thermostable at 50°C. The higher frequency of alkaline amino acids (Arg and Lys), greater pKa values of the catalytic residues, and more positively charged residues on the surface of Man5A might be the causes. Man5A has strong resistance to various neutral and alkaline proteases, retaining more than 97% of the activity after proteolytic treatment for 1 h. The superior characteristics of Man5A make it more advantageous for the application in the kraft pulp industry.
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