ArticleApplied and environmental microbiology2012
How posttranslational modification of nitrogenase is circumvented in Rhodopseudomonas palustris strains that produce hydrogen gas constitutively.
Article in Applied and environmental microbiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 66 citations in OpenAlex.
- Nitrogenase regulation inbioRxiv : the preprint server for biology · 2026Article
- Selected-Wavelength Illumination for Enhanced Hydrogen and Poly-β-hydroxybutyrate Production from Second Cheese Whey byMicroorganisms · 2025Article
- PApplied and environmental microbiology · 2025Article
- Nitrogen fixation in the widely distributed marine γ-proteobacterial diazotrophScience advances · 2024Article
- Rice N-biofertilization by inoculation with an engineered photosynthetic diazotroph.World journal of microbiology & biotechnology · 2024Article
- Complete Genome Sequence of Rhodopseudomonas palustris CGA0092 and Corrections to the R. palustris CGA009 Genome Sequence.Microbiology resource announcements · 2023Article
- Nitrogen Fixation and Ammonium Assimilation Pathway Expression of Geobacter sulfurreducens Changes in Response to the Anode Potential in Microbial Electrochemical Cells.Applied and environmental microbiology · 2023Article
- A Red Fluorescent Protein Reporter System Developed for Measuring Gene Expression in Photosynthetic Bacteria under Anaerobic Conditions.Microorganisms · 2022Article
- Characteristics and Application ofFrontiers in bioengineering and biotechnology · 2022Review
- Potential of Phototrophic Purple Nonsulfur Bacteria to Fix Nitrogen in Rice Fields.Microorganisms · 2021Review
- Reduction of Substrates by Nitrogenases.Chemical reviews · 2020Review
- Rhodobacter capsulatus AnfA is essential for production of Fe-nitrogenase proteins but dispensable for cofactor biosynthesis and electron supply.MicrobiologyOpen · 2020Article
- The Role of Nitric Oxide in Nitrogen Fixation by Legumes.Frontiers in plant science · 2020Review
- NifA is the master regulator of both nitrogenase systems in Rhodobacter capsulatus.MicrobiologyOpen · 2019Article
- Heterotrophic Bacteria Dominate the Diazotrophic Community in the Eastern Indian Ocean (EIO) during Pre-Southwest Monsoon.Microbial ecology · 2019Article
- System-level analysis of metabolic trade-offs during anaerobic photoheterotrophic growth in Rhodopseudomonas palustris.BMC bioinformatics · 2019Article
- Influence of Energy and Electron Availability onApplied and environmental microbiology · 2019Article
- Genome Wide Phosphoproteome Analysis ofFrontiers in microbiology · 2019Article
- Biotransformation of Rutin Using Crude Enzyme from Rhodopseudomonas palustris.Current microbiology · 2017Article
- A Rhizobiales-Specific Unipolar Polysaccharide Adhesin Contributes to Rhodopseudomonas palustris Biofilm Formation across Diverse Photoheterotrophic Conditions.Applied and environmental microbiology · 2017Article
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Nitrogenase catalyzes the conversion of dinitrogen gas (N(2)) and protons to ammonia and hydrogen gas (H(2)). This is a catalytically difficult reaction that requires large amounts of ATP and reducing power. Thus, nitrogenase is not normally expressed or active in bacteria grown with a readily utilized nitrogen source like ammonium. nifA* mutants of the purple nonsulfur phototrophic bacterium Rhodopseudomonas palustris have been described that express nitrogenase genes constitutively and produce H(2) when grown with ammonium as a nitrogen source. This raised the regulatory paradox of why these mutants are apparently resistant to a known posttranslational modification system that should switch off the activity of nitrogenase. Microarray, mutation analysis, and gene expression studies showed that posttranslational regulation of nitrogenase activity in R. palustris depends on two proteins: DraT2, an ADP-ribosyltransferase, and GlnK2, an NtrC-regulated P(II) protein. GlnK2 was not well expressed in ammonium-grown NifA* cells and thus not available to activate the DraT2 nitrogenase modification enzyme. In addition, the NifA* strain had elevated nitrogenase activity due to overexpression of the nif genes, and this increased amount of expression overwhelmed a basal level of activity of DraT2 in ammonium-grown cells. Thus, insufficient levels of both GlnK2 and DraT2 allow H(2) production by an nifA* mutant grown with ammonium. Inactivation of the nitrogenase posttranslational modification system by mutation of draT2 resulted in increased H(2) production by ammonium-grown NifA* cells.
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