ArticleArchives of biochemistry and biophysics2012
Regulation of mouse Cyp24a1 expression via promoter-proximal and downstream-distal enhancers highlights new concepts of 1,25-dihydroxyvitamin D(3) action.
Article in Archives of biochemistry and biophysics, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 47 citations in OpenAlex.
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- Trial
- In Vivo Contribution of Cyp24a1 Promoter Vitamin D Response Elements.Endocrinology · 2024Article
- Article
- 8-Oxoguanine DNA Glycosylase 1 Upregulation as a Risk Factor for Obesity and Colorectal Cancer.International journal of molecular sciences · 2023Article
- Beneficial Effect of Vitamin D on Non-Alcoholic Fatty Liver Disease (NAFLD) Progression in the Zebrafish Model.Nutrients · 2023Article
- Vitamin DInternational journal of molecular sciences · 2022Article
- Vitamin D intake as well as circulating 25-hydroxyvitamin D level and risk for the incidence and recurrence of colorectal cancer precursors: A meta-analysis.Frontiers in medicine · 2022Article
- Vitamin D signaling inhibits HBV activity by directly targeting the HBV core promoter.The Journal of biological chemistry · 2021Article
- Comparative analysis of the association between various serum vitamin D biomarkers and sarcopenia.Journal of clinical laboratory analysis · 2021Article
- Recommendations on the measurement and the clinical use of vitamin D metabolites and vitamin D binding protein - A position paper from the IFCC Committee on bone metabolism.Clinica chimica acta; international journal of clinical chemistry · 2021Review
- Low Prenatal Vitamin D Metabolite Ratio and Subsequent Postpartum Depression Risk.Journal of women's health (2002) · 2021Article
- Serum 24,25-dihydroxyvitamin D level in general Korean population and its relationship with other vitamin D biomarkers.PloS one · 2021Article
- Vitamin DBMC endocrine disorders · 2020Article
- A Narrative Role of Vitamin D and Its Receptor: With Current Evidence on the Gastric Tissues.International journal of molecular sciences · 2019Review
- Article
- Preclinical Prevention Trial of Calcitriol: Impact of Stage of Intervention and Duration of Treatment on Oral Carcinogenesis.Neoplasia (New York, N.Y.) · 2019Article
- A kidney-specific genetic control module in mice governs endocrine regulation of the cytochrome P450 geneThe Journal of biological chemistry · 2017Article
- Article
- LSD1 dual function in mediating epigenetic corruption of the vitamin D signaling in prostate cancer.Clinical epigenetics · 2017Article
Corrections and comments
- Erratum issued
Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
CYP24A1 functions in vitamin D target tissues to degrade 1,25-dihydroxyvitamin D(3) (1,25(OH)(2)D(3)). Thus, the concentration of this enzyme and the regulation of its expression is a primary determinant of the overall biological activity of 1,25(OH)(2)D(3) within cells. The principle regulator of CYP24A1 expression is 1,25(OH)(2)D(3) itself, which functions through the vitamin D receptor to upregulate the transcriptional activity of the Cyp24a1 gene. In this report, we explore the mechanism of this regulation using recently developed ChIP-chip and ChIP-seq techniques that permit an unbiased search for enhancer elements that participate in this transcriptional control. Our studies both confirm a regulatory region defined earlier and located proximal to the transcriptional start site (TSS) of mouse Cyp24a1 (-160 and -265nt) and identify a novel intergenic region located downstream of the transcription unit that contains two enhancers (+35 and +37kb) that facilitate 1,25(OH)(2)D(3)-dependent upregulation of Cyp24a1 expression. Interestingly, while C/EBPβ also binds under basal conditions to a site located immediately upstream of the Cyp24a1 promoter (-345nt), occupancy by this factor is strikingly increased following 1,25(OH)(2)D(3) treatment. The locations and activities of these regulatory regions that mediate 1,25(OH)(2)D(3) actions were confirmed in mice in vivo. We conclude that the mechanism through which 1,25(OH)(2)D(3) induces the CYP24A1 enzyme, thereby autoregulating its own destruction, involves both promoter-proximal as well as downstream-distal enhancers. These findings highlight new concepts regarding the molecular mechanism of action of 1,25(OH)(2)D(3) and other hormonal regulators.
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