ArticleJournal of bacteriology2012
Growth and localization of polyhydroxybutyrate granules in Ralstonia eutropha.
Article in Journal of bacteriology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
27 citing papers in PubMed, 69 citations in OpenAlex.
- Article
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- Magnetosome organelles are organized through interactions between McaA and McaB that alter the dynamics of the bacterial actin-like protein MamK.bioRxiv : the preprint server for biology · 2025Article
- Organizing organelles: bacterial strategies for localizing intracellular compartments.Current opinion in microbiology · 2025Review
- Quantitative Analysis ofBiomolecules · 2024Article
- Three-dimensional label-free visualization and quantification of polyhydroxyalkanoates in individual bacterial cell in its native state.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Synthetic Biology on Acetogenic Bacteria for Highly Efficient Conversion of C1 Gases to Biochemicals.International journal of molecular sciences · 2020Review
- Preparing samples from whole cells using focused-ion-beam milling for cryo-electron tomography.Nature protocols · 2020Article
- Cryo-Electron Tomography Reveals the Complex Ultrastructural Organization of Multicellular FilamentousFrontiers in microbiology · 2020Article
- Simulations suggest a constrictive force is required for Gram-negative bacterial cell division.Nature communications · 2019Article
- Calcium Involved Directional Organization of Polymer Chains in Polyester Nanogranules in Bacterial Cells.Scientific reports · 2019Article
- Poly-3-Hydroxybutyrate Functionalization with BioF-Tagged Recombinant Proteins.Applied and environmental microbiology · 2018Article
- Polyhydroxyalkanoate-associated phasins as phylogenetically heterogeneous, multipurpose proteins.Microbial biotechnology · 2017Review
- Proteins with CHADs (Conserved Histidine α-Helical Domains) Are Attached to Polyphosphate GranulesApplied and environmental microbiology · 2017Article
- Polyhydroxyalkanoate (PHA) Granules Have no Phospholipids.Scientific reports · 2016Article
- A new view into prokaryotic cell biology from electron cryotomography.Nature reviews. Microbiology · 2016Review
- Formation of polyphosphate by polyphosphate kinases and its relationship to poly(3-hydroxybutyrate) accumulation in Ralstonia eutropha strain H16.Applied and environmental microbiology · 2015Article
- Comparative proteome analysis reveals four novel polyhydroxybutyrate (PHB) granule-associated proteins in Ralstonia eutropha H16.Applied and environmental microbiology · 2015Article
- Ultrastructure and complex polar architecture of the human pathogen Campylobacter jejuni.MicrobiologyOpen · 2014Article
- PhaM is the physiological activator of poly(3-hydroxybutyrate) (PHB) synthase (PhaC1) in Ralstonia eutropha.Applied and environmental microbiology · 2014Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
The bacterium Ralstonia eutropha forms cytoplasmic granules of polyhydroxybutyrate that are a source of biodegradable thermoplastic. While much is known about the biochemistry of polyhydroxybutyrate production, the cell biology of granule formation and growth remains unclear. Previous studies have suggested that granules form either in the inner membrane, on a central scaffold, or in the cytoplasm. Here we used electron cryotomography to monitor granule genesis and development in 3 dimensions (3-D) in a near-native, "frozen-hydrated" state in intact Ralstonia eutropha cells. Neither nascent granules within the cell membrane nor scaffolds were seen. Instead, granules of all sizes resided toward the center of the cytoplasm along the length of the cell and exhibited a discontinuous surface layer more consistent with a partial protein coating than either a lipid mono- or bilayer. Putatively fusing granules were also seen, suggesting that small granules are continually generated and then grow and merge. Together, these observations support a model of biogenesis wherein granules form in the cytoplasm coated not by phospholipid but by protein. Previous thin-section electron microscopy (EM), fluorescence microscopy, and atomic force microscopy (AFM) results to the contrary may reflect both differences in nucleoid condensation and specimen preparation-induced artifacts.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.