ArticleJournal of bacteriology2012
Membrane topology of the Bacillus anthracis GerH germinant receptor proteins.
Article in Journal of bacteriology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 30 citations in OpenAlex.
- Structural and functional analysis of themBio · 2026Article
- GerAB residues predicted to interfere with water passage based on steered molecular dynamics are key to germinosome functionality.Frontiers in microbiology · 2025Article
- Bacterial spore germination receptors are nutrient-gated ion channels.Science (New York, N.Y.) · 2023Article
- Genetic Suppression Meets Structure Prediction: Probing a Spore Germination Receptor Complex.Journal of bacteriology · 2022Article
- Dormant spores sense amino acids through the B subunits of their germination receptors.Nature communications · 2021Article
- Predicting the Structure and Dynamics of Membrane Protein GerAB fromInternational journal of molecular sciences · 2021Article
- Visualization of Germination Proteins in PutativeInternational journal of molecular sciences · 2020Article
- Structural and functional analyses of the N-terminal domain of the A subunit of aProceedings of the National Academy of Sciences of the United States of America · 2019Article
- DdcA antagonizes a bacterial DNA damage checkpoint.Molecular microbiology · 2019Article
- Positions 299 and 302 of the GerAA subunit are important for function of the GerA spore germination receptor in Bacillus subtilis.PloS one · 2018Article
- Petrobactin Is Exported frommBio · 2017Article
- Reoxidation of the Thiol-Disulfide Oxidoreductase MdbA by a Bacterial Vitamin K Epoxide Reductase in the Biofilm-Forming Actinobacterium Actinomyces oris.Journal of bacteriology · 2017Article
- L-alanine-induced germination in Bacillus licheniformis -the impact of native gerA sequences.BMC microbiology · 2014Article
- Germination of spores of Bacillus species: what we know and do not know.Journal of bacteriology · 2014Review
- Numbers of individual nutrient germinant receptors and other germination proteins in spores of Bacillus subtilis.Journal of bacteriology · 2013Article
- Topology and accessibility of germination proteins in the Bacillus subtilis spore inner membrane.Journal of bacteriology · 2013Article
- Multiple ABC transporters are involved in the acquisition of petrobactin in Bacillus anthracis.Molecular microbiology · 2012Article
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Authors and funding
4 authors at 1 institution in 1 country.
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Abstract
Bacillus anthracis spores are the etiologic agent of anthrax. Nutrient germinant receptors (nGRs) packaged within the inner membrane of the spore sense the presence of specific stimuli in the environment and trigger the process of germination, quickly returning the bacterium to the metabolically active, vegetative bacillus. This ability to sense the host environment and initiate germination is a required step in the infectious cycle. The nGRs are comprised of three subunits: the A-, B-, and C-type proteins. To date there are limited structural data for the A- and B-type nGR subunits. Here the transmembrane topologies of the B. anthracis GerH(A), GerH(B), and GerH(C) proteins are presented. C-terminal green fluorescent protein (GFP) fusions to various lengths of the GerH proteins were overexpressed in vegetative bacteria, and the subcellular locations of these GFP fusion sites were analyzed by flow cytometry and protease sensitivity. GFP fusion to full-length GerH(C) confirmed that the C terminus of this protein is extracellular, as predicted. GerH(A) and GerH(B) were both predicted to be integral membrane proteins by topology modeling. Analysis of C-terminal GFP fusions to full-length GerH(B) and nine truncated GerH(B) proteins supports either an 8- or 10-transmembrane-domain topology. For GerH(A), C-terminal GFP fusions to full-length GerH(A) and six truncated GerH(A) proteins were consistent with a four-transmembrane-domain topology. Understanding the membrane topology of these proteins is an important step in determining potential ligand binding and protein-protein interaction domains, as well as providing new information for interpreting previous genetic work.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.