ArticleJournal of molecular biology2012
A structural basis for sustained bacterial adhesion: biomechanical properties of CFA/I pili.
Article in Journal of molecular biology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
20 citing papers in PubMed, 45 citations in OpenAlex.
- Endospore pili: Flexible, stiff, and sticky nanofibers.Biophysical journal · 2023Article
- Three structural solutions for bacterial adhesion pilus stability and superelasticity.Structure (London, England : 1993) · 2023Article
- The F-pilus biomechanical adaptability accelerates conjugative dissemination of antimicrobial resistance and biofilm formation.Nature communications · 2023Article
- Unveiling molecular interactions that stabilize bacterial adhesion pili.Biophysical journal · 2022Article
- Impact of an alpha helix and a cysteine-cysteine disulfide bond on the resistance of bacterial adhesion pili to stress.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Chaperone-tip adhesin complex is vital for synergistic activation of CFA/I fimbriae biogenesis.PLoS pathogens · 2020Article
- Cryo-EM structure of the CFA/I pilus rod.IUCrJ · 2019Article
- A Role for Salivary Peptides in the Innate Defense Against Enterotoxigenic Escherichia coli.The Journal of infectious diseases · 2018Article
- Antibodies Damage the Resilience of Fimbriae, Causing Them To Be Stiff and Tangled.Journal of bacteriology · 2017Article
- Antibody-mediated disruption of the mechanics of CS20 fimbriae of enterotoxigenic Escherichia coli.Scientific reports · 2015Article
- Biomechanical and structural features of CS2 fimbriae of enterotoxigenic Escherichia coli.Biophysical journal · 2015Article
- Rigid multibody simulation of a helix-like structure: the dynamics of bacterial adhesion pili.European biophysics journal : EBJ · 2015Article
- The intestinal microbiota: its role in health and disease.European journal of pediatrics · 2015Review
- Structure and function of enterotoxigenic Escherichia coli fimbriae from differing assembly pathways.Molecular microbiology · 2015Article
- Yielding elastic tethers stabilize robust cell adhesion.PLoS computational biology · 2014Article
- Virulence regulons of enterotoxigenic Escherichia coli.Immunologic research · 2013Review
- The shaft of the type 1 fimbriae regulates an external force to match the FimH catch bond.Biophysical journal · 2013Article
- Tight conformational coupling between the domains of the enterotoxigenic Escherichia coli fimbrial adhesin CfaE regulates binding state transition.The Journal of biological chemistry · 2013Article
- The structure of the CS1 pilus of enterotoxigenic Escherichia coli reveals structural polymorphism.Journal of bacteriology · 2013Article
- Helix-like biopolymers can act as dampers of force for bacteria in flows.European biophysics journal : EBJ · 2012Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
Abstract
Enterotoxigenic Escherichia coli (ETEC) are a major cause of diarrheal disease worldwide. Adhesion pili (or fimbriae), such as the CFA/I (colonization factor antigen I) organelles that enable ETEC to attach efficiently to the host intestinal tract epithelium, are critical virulence factors for initiation of infection. We characterized the intrinsic biomechanical properties and kinetics of individual CFA/I pili at the single-organelle level, demonstrating that weak external forces (7.5 pN) are sufficient to unwind the intact helical filament of this prototypical ETEC pilus and that it quickly regains its original structure when the force is removed. While the general relationship between exertion of force and an increase in the filament length for CFA/I pili associated with diarrheal disease is analogous to that of P pili and type 1 pili, associated with urinary tract and other infections, the biomechanical properties of these different pili differ in key quantitative details. Unique features of CFA/I pili, including the significantly lower force required for unwinding, the higher extension speed at which the pili enter a dynamic range of unwinding, and the appearance of sudden force drops during unwinding, can be attributed to morphological features of CFA/I pili including weak layer-to-layer interactions between subunits on adjacent turns of the helix and the approximately horizontal orientation of pilin subunits with respect to the filament axis. Our results indicate that ETEC CFA/I pili are flexible organelles optimized to withstand harsh motion without breaking, resulting in continued attachment to the intestinal epithelium by the pathogenic bacteria that express these pili.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.