ArticleJournal of structural biology2012
Cryo X-ray nano-tomography of vaccinia virus infected cells.
Article in Journal of structural biology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 96 citations in OpenAlex.
- 3D Cryo-Correlative Methods to Study Virus Structure and Dynamics Within Cells.Sub-cellular biochemistry · 2024Review
- 3D surface reconstruction of cellular cryo-soft X-ray microscopy tomograms using semisupervised deep learning.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Correlative Cryo-imaging Using Soft X-Ray Tomography for the Study of Virus Biology in Cells and Tissues.Sub-cellular biochemistry · 2023Article
- In Situ Imaging of Virus-Infected Cells by Cryo-Electron Tomography: An Overview.Sub-cellular biochemistry · 2023Review
- Near-native state imaging by cryo-soft-X-ray tomography reveals remodelling of multiple cellular organelles during HSV-1 infection.PLoS pathogens · 2022Article
- A guide into the world of high-resolution 3D imaging: the case of soft X-ray tomography for the life sciences.Biochemical Society transactions · 2022Article
- Soft X-ray tomography to map and quantify organelle interactions at the mesoscale.Structure (London, England : 1993) · 2022Article
- Correlative 3D cryo X-ray imaging reveals intracellular location and effect of designed antifibrotic protein-nanomaterial hybrids.Chemical science · 2021Article
- Using soft X-ray tomography for rapid whole-cell quantitative imaging of SARS-CoV-2-infected cells.Cell reports methods · 2021Article
- Advances and insights in the diagnosis of viral infections.Journal of nanobiotechnology · 2021Review
- Review
- Quantitative conversion of biomass in giant DNA virus infection.Scientific reports · 2021Article
- Four-Dimensional Characterization of themSphere · 2020Article
- Article
- The achievable resolution for X-ray imaging of cells and other soft biological material.IUCrJ · 2020Article
- The cellular landscape by cryo soft X-ray tomography.Biophysical reviews · 2019Review
- Imaging cell morphology and physiology using X-rays.Biochemical Society transactions · 2019Review
- Cryo-soft X-ray tomography: using soft X-rays to explore the ultrastructure of whole cells.Emerging topics in life sciences · 2018Article
- Visualizing red blood cell sickling and the effects of inhibition of sphingosine kinase 1 using soft X-ray tomography.Journal of cell science · 2016Article
- Cryo-soft X-ray tomography as a quantitative three-dimensional tool to model nanoparticle:cell interaction.Journal of nanobiotechnology · 2016Article
Corrections and comments
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Authors and funding
11 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have performed full-field cryo X-ray microscopy in the water window photon energy range on vaccinia virus (VACV) infected cells to produce tomographic reconstructions. PtK2 cells were infected with a GFP-expressing VACV strain and frozen by plunge fast freezing. The infected cells were selected by light fluorescence microscopy of the GFP marker and subsequently imaged in the X-ray microscope under cryogenic conditions. Tomographic tilt series of X-ray images were used to yield three-dimensional reconstructions showing different cell organelles (nuclei, mitochondria, filaments), together with other structures derived from the virus infection. Among them, it was possible to detect viral factories and two types of viral particles related to different maturation steps of VACV (immature and mature particles), which were compared to images obtained by standard electron microscopy of the same type of cells. In addition, the effect of radiation damage during X-ray tomographic acquisition was analyzed. Thin sections studied by electron microscopy revealed that the morphological features of the cells do not present noticeable changes after irradiation. Our findings show that cryo X-ray nano-tomography is a powerful tool for collecting three-dimensional structural information from frozen, unfixed, unstained whole cells with sufficient resolution to detect different virus particles exhibiting distinct maturation levels.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.