ArticleBMC biotechnology2011
Real-time PCR-based assay to quantify the relative amount of human and mouse tissue present in tumor xenografts.
Article in BMC biotechnology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers.
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Who cites it
48 citing papers in PubMed.
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- Homeostatic membrane tension constrains cancer cell dissemination by counteracting BAR protein assembly.Nature communications · 2021Article
- Metabolic and transcriptomic profiles of glioblastoma invasion revealed by comparisons between patients and corresponding orthotopic xenografts in mice.Acta neuropathologica communications · 2021Article
- Epicardial placement of human MSC-loaded fibrin sealant films for heart failure: Preclinical efficacy and mechanistic data.Molecular therapy : the journal of the American Society of Gene Therapy · 2021Article
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- Human-specific GAPDH qRT-PCR is an accurate and sensitive method of xenograft metastasis quantification.Molecular therapy. Methods & clinical development · 2021Article
- TMEM52B suppression promotes cancer cell survival and invasion through modulating E-cadherin stability and EGFR activity.Journal of experimental & clinical cancer research : CR · 2021Article
- PDX-Derived Ewing's Sarcoma Cells Retain High Viability and Disease Phenotype in Alginate Encapsulated Spheroid Cultures.Cancers · 2021Article
- Epigenomic Reprogramming toward Mesenchymal-Epithelial Transition in Ovarian-Cancer-Associated Mesenchymal Stem Cells Drives Metastasis.Cell reports · 2020Article
- Systemic cancer therapy with engineered adenovirus that evades innate immunity.Science translational medicine · 2020Article
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Authors and funding
6 authors.
Funding
Abstract
backgroundXenograft samples used to test anti-cancer drug efficacies and toxicities in vivo contain an unknown mix of mouse and human cells. Evaluation of drug activity can be confounded by samples containing large amounts of contaminating mouse tissue. We have developed a real-time quantitative polymerase chain reaction (qPCR) assay using TaqMan technology to quantify the amount of mouse tissue that is incorporated into human xenograft samples.
resultsThe forward and reverse primers bind to the same DNA sequence in the human and the mouse genome. Using a set of specially designed fluorescent probes provides species specificity. The linearity and sensitivity of the assay is evaluated using serial dilutions of single species and heterogeneous DNA mixtures. We examined many xenograft samples at various in vivo passages, finding a wide variety of human:mouse DNA ratios. This variation may be influenced by tumor type, number of serial passages in vivo, and even which part of the tumor was collected and used in the assay.
conclusionsThis novel assay provides an accurate quantitative assessment of human and mouse content in xenograft tumors. This assay can be performed on aberrantly behaving human xenografts, samples used in bioinformatics studies, and periodically for tumor tissue frequently grown by serial passage in vivo.
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