ArticleMolecular plant pathology2012
A secreted lipolytic enzyme from Xanthomonas campestris pv. vesicatoria is expressed in planta and contributes to its virulence.
Article in Molecular plant pathology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 28 citations in OpenAlex.
- Beneath the Bark: Molecular Determinants of Adaptation and Infection in Bacterial Pathogens of Woody Hosts.Molecular plant pathology · 2026Review
- Comparative genomics of strains of varying virulence of the gram-positive bacterial pathogen of maize, Clavibacter nebraskensis.BMC genomics · 2026Article
- Comprehensive Genomics and Functional Validation Reveal Evolutionary Insights into Xanthan Gum Biosynthesis and Pathogenesis ofJournal of agricultural and food chemistry · 2026Article
- Functional Cargo in Membrane Vesicles From a Citrus Pathogen.Environmental microbiology reports · 2025Article
- Comparative transcriptomics reveals a highly polymorphic Xanthomonas HrpG virulence regulon.BMC genomics · 2024Article
- The GDSL-Lipolytic Enzyme Lip1 Is Required for Full Virulence of the Cucurbit Pathogenic BacteriumMicroorganisms · 2022Article
- Looking through the FOG: microbiome characterization and lipolytic bacteria isolation from a fatberg site.Microbiology (Reading, England) · 2021Article
- The HrpG/HrpX Regulon of Xanthomonads-An Insight to the Complexity of Regulation of Virulence Traits in Phytopathogenic Bacteria.Microorganisms · 2021Review
- Identification and analysis of seven effector protein families with different adaptive and evolutionary histories in plant-associated members of the Xanthomonadaceae.Scientific reports · 2017Article
- Outer membrane vesicle-associated lipase FtlA enhances cellular invasion and virulence in Francisella tularensis LVS.Emerging microbes & infections · 2017Article
- Bacterial pathogenesis of plants: future challenges from a microbial perspective: Challenges in Bacterial Molecular Plant Pathology.Molecular plant pathology · 2016Review
- Understanding the direction of evolution in Burkholderia glumae through comparative genomics.Current genetics · 2016Review
- The Type II Secreted Lipase/Esterase LesA is a Key Virulence Factor Required for Xylella fastidiosa Pathogenesis in Grapevines.Scientific reports · 2016Article
- Bacterial spot of tomato and pepper: diverse Xanthomonas species with a wide variety of virulence factors posing a worldwide challenge.Molecular plant pathology · 2015Article
- Xanthomonas campestris pv. vesicatoria Secretes Proteases and Xylanases via the Xps Type II Secretion System and Outer Membrane Vesicles.Journal of bacteriology · 2015Article
- Secreted fungal effector lipase releases free fatty acids to inhibit innate immunity-related callose formation during wheat head infection.Plant physiology · 2014Article
- The role of bacterial biofilms and surface components in plant-bacterial associations.International journal of molecular sciences · 2013Review
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A recombinase-based in vivo expression technology (RIVET) approach with Xanthomonas campestris pv. vesicatoria (Xcv) revealed that lipA, annotated as putative secreted lipase, is expressed during the interaction between this pathogen and tomato. Here, the tnpR and uidA reporter genes were used to show that lipA is strongly induced in XVM2 minimal medium and during the early stages of tomato infection by Xcv. A mutant strain impaired in lipA was generated by insertional mutagenesis. This mutant grew in a similar manner to the wild-type in rich medium, but its growth was significantly compromised in a medium containing olive oil as a single carbon source. The lipolytic activity of the extracellular fraction of the lipA mutant was reduced significantly relative to that of the wild-type strain, thus confirming that lipA indeed encodes a functional secreted enzyme with lipolytic activity. A plasmid carrying a wild-type copy of lipA complemented the lipA mutant for extracellular lipolytic activity. Dip inoculation experiments with tomato lines Hawaii 7998 (H7998) and Micro Tom showed that the lipA mutant grew to a lesser extent than the wild-type in tomato leaves. Following leaf syringe infiltrations, the mutant strain induced disease symptoms that were less severe than those induced by the wild-type strain, supporting a significant role of lipA in the pathogenicity of Xcv.
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