ArticleJournal of the American Chemical Society2012
Diels-Alder cycloaddition for fluorophore targeting to specific proteins inside living cells.
Article in Journal of the American Chemical Society, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 89 papers.
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Who cites it
89 citing papers in PubMed.
- Harnessing fluorescent probes for molecular diagnosis and theranostics of atherosclerosis.Drug delivery · 2026Review
- Tetrazine-Inorganic chemistry frontiers · 2026Article
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- Tuning Encodable Tetrazine Chemistry for Site-Specific Protein Bioorthogonal Ligations.Angewandte Chemie (International ed. in English) · 2025Article
- Positive-going hybrid indicators for voltage imaging in excitable cells and tissues.Science advances · 2025Article
- Investigation of Proteome-Tetrazine Reactivity for a Highly Selective Tetrazine Ligation in Live Cells.ACS central science · 2025Article
- Bio-orthogonal tuning of matrix properties during 3D cell culture to induce morphological and phenotypic changes.Nature protocols · 2025Review
- Enhanced optical imaging and fluorescent labeling for visualizing drug molecules within living organisms.Acta pharmaceutica Sinica. B · 2024Review
- Advances in the Synthesis of Bioorthogonal Reagents: s-Tetrazines, 1,2,4-Triazines, Cyclooctynes, Heterocycloheptynes, and trans-Cyclooctenes.Topics in current chemistry (Cham) · 2024Review
- Readily Accessible and Brightly Fluorogenic BODIPY/NBD-Tetrazines via SThe Journal of organic chemistry · 2024Article
- Engineering of cell-surface receptors for analysis of receptor internalization and detection of receptor-specific glycosylation.Chemical science · 2024Article
- Promiscuous Enzymes for Residue-Specific Peptide and Protein Late-Stage Functionalization.Chembiochem : a European journal of chemical biology · 2023Review
- Supertemporal Resolution Imaging of Membrane Potential via Stroboscopic Microscopy.Chemical & biomedical imaging · 2023Article
- Orange/far-red hybrid voltage indicators with reduced phototoxicity enable reliable long-term imaging in neurons and cardiomyocytes.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Article
- Proximity-enhanced protein crosslinking through an alkene-tetrazine reaction.Bioorganic chemistry · 2023Article
- HIV infection detection using CRISPR/Cas systems: Present and future prospects.Computational and structural biotechnology journal · 2023Review
- Functionalization of Radiolabeled Antibodies to Enhance Peripheral Clearance for High Contrast Brain Imaging.Molecular pharmaceutics · 2022Article
- Further assessments of ligase LplA-mediated modifications of proteins in vitro and in cellulo.Molecular biology reports · 2022Article
- A far-red hybrid voltage indicator enabled by bioorthogonal engineering of rhodopsin on live neurons.Nature chemistry · 2021Article
29 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
The inverse-electron-demand Diels-Alder cycloaddition between trans-cyclooctenes and tetrazines is biocompatible and exceptionally fast. We utilized this chemistry for site-specific fluorescence labeling of proteins on the cell surface and inside living mammalian cells by a two-step protocol. Escherichia coli lipoic acid ligase site-specifically ligates a trans-cyclooctene derivative onto a protein of interest in the first step, followed by chemoselective derivatization with a tetrazine-fluorophore conjugate in the second step. On the cell surface, this labeling was fluorogenic and highly sensitive. Inside the cell, we achieved specific labeling of cytoskeletal proteins with green and red fluorophores. By incorporating the Diels-Alder cycloaddition, we have broadened the panel of fluorophores that can be targeted by lipoic acid ligase.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.