ArticlePloS one2011
Generation of stratified squamous epithelial progenitor cells from mouse induced pluripotent stem cells.
Article in PloS one, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers, 1 of them a synthesis that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
22 citing papers in PubMed, 1 synthesis or guideline pooled it, 35 citations in OpenAlex.
- A Potential Concomitant Sellar Embryonic Remnant-Associated Collision Tumor: Systematic Review.Frontiers in oncology · 2021Pooled it
- Evaluation of the Therapeutic Efficacy of Stromal Cells Isolated from Human Corneal Lenticules for Corneal Repair after Injury in an In Vivo Model.Bulletin of experimental biology and medicine · 2025Article
- 3D bioprinting as a prospective therapeutic strategy for corneal limbal epithelial stem cell deficiency.International journal of bioprinting · 2023Article
- The effects of continuous exposure to low-dose chlorine dioxide gas on the characteristics of induced pluripotent stem cells.Regenerative therapy · 2022Article
- Article
- Regenerative therapy for the Cornea.Progress in retinal and eye research · 2022Review
- Corneal epithelium in keratoconus underexpresses active NRF2 and a subset of oxidative stress-related genes.PloS one · 2022Article
- DNCP induces the differentiation of induced pluripotent stem cells into odontoblasts by activating the Smad/p-Smad and p38/p-p38 signaling pathways.Experimental and therapeutic medicine · 2021Article
- Head and Neck Cancer Stem Cell-Enriched Spheroid Model for Anticancer Compound Screening.Cells · 2020Article
- Corneal Stem Cells as a Source of Regenerative Cell-Based Therapy.Stem cells international · 2020Review
- Human induced pluripotent stem cell-derived vocal fold mucosa mimics development and responses to smoke exposure.Nature communications · 2019Article
- Circadian rhythms, metabolic oscillators, and the target of rapamycin (TOR) pathway: the Neurospora connection.Current genetics · 2019Review
- A subtype of cancer-associated fibroblasts with lower expression of alpha-smooth muscle actin suppresses stemness through BMP4 in oral carcinoma.Oncogenesis · 2018Article
- Histological subtypes of mouse mammary tumors reveal conserved relationships to human cancers.PLoS genetics · 2018Article
- The promise of stem cell-based therapeutics in ophthalmology.Neural regeneration research · 2017Review
- Progress in corneal wound healing.Progress in retinal and eye research · 2015Review
- Differentiation of human limbal-derived induced pluripotent stem cells into limbal-like epithelium.Stem cells translational medicine · 2014Article
- iPS cell technologies: significance and applications to CNS regeneration and disease.Molecular brain · 2014Review
- Differentiation potential of limbal fibroblasts and bone marrow mesenchymal stem cells to corneal epithelial cells.Stem cells (Dayton, Ohio) · 2014Article
- Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundApplication of induced pluripotent stem (iPS) cells in regenerative medicine will bypass ethical issues associated with use of embryonic stem cells. In addition, patient-specific IPS cells can be useful to elucidate the pathophysiology of genetic disorders, drug screening, and tailor-made medicine. However, in order to apply iPS cells to mitotic tissue, induction of tissue stem cells that give rise to progeny of the target organ is required. METHODOLOGY/PRINCIPAL
findingsWe induced stratified epithelial cells from mouse iPS cells by co-culture with PA6 feeder cells (SDIA-method) with use of BMP4. Clusters of cells positive for the differentiation markers KRT1 or KRT12 were observed in KRT14-positive colonies. We successfully cloned KRT14 and p63 double-positive stratified epithelial progenitor cells from iPS-derived epithelial cells, which formed stratified epithelial sheets consisting of five- to six-polarized epithelial cells in vitro. When these clonal cells were cultured on denuded mouse corneas, a robust stratified epithelial layer was observed with physiological cell polarity including high levels of E-cadherin, p63 and K15 expression in the basal layer and ZO-1 in the superficial layer, recapitulating the apico-basal polarity of the epithelium in vivo. CONCLUSIONS/SIGNIFICANCE: These results suggest that KRT14 and p63 double-positive epithelial progenitor cells can be cloned from iPS cells in order to produce polarized multilayer epithelial cell sheets.
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Registered trials
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