ArticlePloS one2011
Erwinia amylovora novel plasmid pEI70: complete sequence, biogeography, and role in aggressiveness in the fire blight phytopathogen.
Article in PloS one, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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17 citing papers in PubMed.
- Mutation of Eamy_RS26425,Applied and environmental microbiology · 2026Article
- Intraspecific diversity of Erwinia amylovora strains from northern Algeria.BMC microbiology · 2024Article
- Comparative genomics to examine the endophytic potential of Pantoea agglomerans DAPP-PG 734.BMC genomics · 2022Article
- Genome sequencing provides new insights on the distribution of Erwinia amylovora lineages in northern Italy.Environmental microbiology reports · 2022Article
- Priority Effects in the Apple Flower Determine If the Siderophore Desferrioxamine Is a Virulence Factor for Erwinia amylovora CFBP1430.Applied and environmental microbiology · 2022Article
- Spontaneous Resistance ofFrontiers in microbiology · 2022Article
- CRISPR genotyping as complementary tool for epidemiological surveillance of Erwinia amylovora outbreaks.PloS one · 2021Article
- Development of a real-time PCR method for the specific detection of the novel pear pathogen Erwinia uzenensis.PloS one · 2019Article
- Comparative genomics of Spiraeoideae-infecting Erwinia amylovora strains provides novel insight to genetic diversity and identifies the genetic basis of a low-virulence strain.Molecular plant pathology · 2018Article
- Comparative Genomics Analysis of Plasmid pPV989-94 from a Clinical Isolate ofInternational journal of genomics · 2018Article
- Article
- Genetic islands in pome fruit pathogenic and non-pathogenic Erwinia species and related plasmids.Frontiers in microbiology · 2015Review
- Integrative conjugative elements of the ICEPan family play a potential role in Pantoea ananatis ecological diversification and antibiosis.Frontiers in microbiology · 2015Article
- Conventional and real-time PCRs for detection of Erwinia piriflorinigrans allow its distinction from the fire blight pathogen, Erwinia amylovora.Applied and environmental microbiology · 2014Article
- Comparative genomics of 12 strains of Erwinia amylovora identifies a pan-genome with a large conserved core.PloS one · 2013Article
- Erwinia amylovora CRISPR elements provide new tools for evaluating strain diversity and for microbial source tracking.PloS one · 2012Article
- Functions and origin of plasmids inTrees (Berlin, Germany : West)Review
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11 authors.
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No grant is acknowledged in the PubMed record.
Abstract
Comparative genomics of several strains of Erwinia amylovora, a plant pathogenic bacterium causal agent of fire blight disease, revealed that its diversity is primarily attributable to the flexible genome comprised of plasmids. We recently identified and sequenced in full a novel 65.8 kb plasmid, called pEI70. Annotation revealed a lack of known virulence-related genes, but found evidence for a unique integrative conjugative element related to that of other plant and human pathogens. Comparative analyses using BLASTN showed that pEI70 is almost entirely included in plasmid pEB102 from E. billingiae, an epiphytic Erwinia of pome fruits, with sequence identities superior to 98%. A duplex PCR assay was developed to survey the prevalence of plasmid pEI70 and also that of pEA29, which had previously been described in several E. amylovora strains. Plasmid pEI70 was found widely dispersed across Europe with frequencies of 5-92%, but it was absent in E. amylovora analyzed populations from outside of Europe. Restriction analysis and hybridization demonstrated that this plasmid was identical in at least 13 strains. Curing E. amylovora strains of pEI70 reduced their aggressiveness on pear, and introducing pEI70 into low-aggressiveness strains lacking this plasmid increased symptoms development in this host. Discovery of this novel plasmid offers new insights into the biogeography, evolution and virulence determinants in E. amylovora.
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