ArticlePloS one2011
A method for the direct identification of differentiating muscle cells by a fluorescent mitochondrial dye.
Article in PloS one, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 41 citations in OpenAlex.
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- Principal protocols for the processing of cultured meat.Journal of animal science and technology · 2021Review
- Bioinspired electrospun dECM scaffolds guide cell growth and control the formation of myotubes.Science advances · 2021Article
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- Gain-of-Function STIM1 L96V Mutation Causes Myogenesis Alteration in Muscle Cells From a Patient Affected by Tubular Aggregate Myopathy.Frontiers in cell and developmental biology · 2021Article
- Advances in biomaterials for skeletal muscle engineering and obstacles still to overcome.Materials today. Bio · 2020Review
- Sestrins are evolutionarily conserved mediators of exercise benefits.Nature communications · 2020Article
- Actomyosin contractility scales with myoblast elongation and enhances differentiation through YAP nuclear export.Scientific reports · 2019Article
- Cellular metabolic rates and oxidative stress profiles in primary fibroblast cells isolated from virgin females, reproductively experienced females, and male Sprague-Dawley rats.Physiological reports · 2018Article
- Sphingosine-1-phosphate pretreatment amends hypoxia-induced metabolic dysfunction and impairment of myogenic potential in differentiating C2C12 myoblasts by stimulating viability, calcium homeostasis and energy generation.The journal of physiological sciences : JPS · 2018Article
- Three-dimensional imaging reveals endo(sarco)plasmic reticulum-containing invaginations within the nucleoplasm of muscle.American journal of physiology. Cell physiology · 2018Article
- Metabolic effects of physiological levels of caffeine in myotubes.Journal of physiology and biochemistry · 2018Article
- Graphene Foam as a three-dimensional Platform for Myotube Growth.ACS biomaterials science & engineering · 2016Article
- Low glucose but not galactose enhances oxidative mitochondrial metabolism in C2C12 myoblasts and myotubes.PloS one · 2013Article
- Thimerosal-induced apoptosis in mouse C2C12 myoblast cells occurs through suppression of the PI3K/Akt/survivin pathway.PloS one · 2012Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
Identification of differentiating muscle cells generally requires fixation, antibodies directed against muscle specific proteins, and lengthy staining processes or, alternatively, transfection of muscle specific reporter genes driving GFP expression. In this study, we examined the possibility of using the robust mitochondrial network seen in maturing muscle cells as a marker of cellular differentiation. The mitochondrial fluorescent tracking dye, MitoTracker, which is a cell-permeable, low toxicity, fluorescent dye, allowed us to distinguish and track living differentiating muscle cells visually by epi-fluorescence microscopy. MitoTracker staining provides a robust and simple detection strategy for living differentiating cells in culture without the need for fixation or biochemical processing.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.