ArticlePloS one2011
Galectin-1 is part of human trophoblast invasion machinery--a functional study in vitro.
Article in PloS one, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed, 71 citations in OpenAlex.
- Maternal and placental galectins: key players in the feto-maternal symbiotic tango.Seminars in immunopathology · 2025Review
- Galectin-8 Contributes to Human Trophoblast Cell Invasion.International journal of molecular sciences · 2024Article
- Proteomic Analysis of Domestic Cat Blastocysts and Their Secretome Produced in an In Vitro Culture System without the Presence of the Zona Pellucida.International journal of molecular sciences · 2024Article
- Galectin-1 Modulates the Fusogenic Activity of Placental Endogenous Retroviral Envelopes.Viruses · 2023Article
- Trophoblast Cell Function in the Antiphospholipid Syndrome.Biomedicines · 2023Review
- Roles of N-linked glycosylation and glycan-binding proteins in placentation: trophoblast infiltration, immunomodulation, angiogenesis, and pathophysiology.Biochemical Society transactions · 2023Review
- Galectins in Equine Placental Disease.Veterinary sciences · 2023Article
- Galectinology of Equine Pregnancy.Animals : an open access journal from MDPI · 2022Article
- Review
- Galectins: Important Regulators in Normal and Pathologic Pregnancies.International journal of molecular sciences · 2022Review
- Examination of the Contributions of Maternal/Placental-Derived Galectin-1 to Pregnancy Outcome.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Galectins in Early Pregnancy and Pregnancy-Associated Pathologies.International journal of molecular sciences · 2021Review
- The Role of the Adhesion Receptor CD146 and Its Soluble Form in Human Embryo Implantation and Pregnancy.Frontiers in immunology · 2021Review
- Medawar's PostEra: Galectins Emerged as Key Players During Fetal-Maternal Glycoimmune Adaptation.Frontiers in immunology · 2021Review
- Glycan characterization of pregnancy-specific glycoprotein 1 and its identification as a novel Galectin-1 ligand.Glycobiology · 2020Article
- Differential gene expression elicited by ZIKV infection in trophoblasts from congenital Zika syndrome discordant twins.PLoS neglected tropical diseases · 2020Article
- Role of galectin-glycan circuits in reproduction: from healthy pregnancy to preterm birth (PTB).Seminars in immunopathology · 2020Review
- Altered Glycosylation Contributes to Placental Dysfunction Upon Early Disruption of the NK Cell-DC Dynamics.Frontiers in immunology · 2020Article
- Human trophoblast requires galectin-3 for cell migration and invasion.Scientific reports · 2019Article
- Pregnancy Galectinology: Insights Into a Complex Network of Glycan Binding Proteins.Frontiers in immunology · 2019Review
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundInteractions of glycoconjugates with endogenous galectins, have been long proposed to participate in several reproductive processes including implantation. In human placenta gal-1, gal-3, gal-8, and gal-13 proteins are known to be present. Each of them has been proposed to play multiple functions, but so far no clear picture has emerged. We hypothesized that gal-1 participates in trophoblast invasion, and conducted Matrigel invasion assay using isolated cytotrophoblast from first trimester placenta and HTR-8/SVneo cell line to test it. METHODS AND
findingsFunction blocking anti-gal-1 antibody was employed to assess participation of endogenous gal-1 in cell adhesion, cell invasion of HTR-8/SVneo cells. When gal-1 was blocked in isolated trophoblast cell invasion was reduced to 75% of control (SEM ± 6.3, P<0.001) and to 66% of control (SEM ± 1.7, P<0.001) in HTR-8/SVneo cell line. Increased availability of gal-1, as two molecular forms of recombinant human gal-1 (CS-gal-1 and Ox-gal-1), resulted in increased cell invasion by cytotrophoblast to 151% (SEM ± 16, P<0.01) with 1 ng/ml of CS-gal-1, and to 192% (SEM ± 51, P<0.05) with 1 µg/ml of Ox-gal-1. Stimulation was also observed in HTR-8/SVneo cells, to 317% (SEM ± 58, P<0.001) by CS-gal-1, and to 200% (SEM ± 24, P<0.001) by Ox-gal-1 at 1 µg/ml. Both sets of results confirmed involvement of gal-1 in trophoblast invasion. Galectin profile of isolated cytotrophoblast and HTR-8/SVneo cells was established using RT-PCR and real-time PCR and found to consist of gal-1, gal-3 and gal-8 for both cell types. Only gal-1 was located at the trophoblast cell membrane, as determined by FACS analysis, which is consistent with the results of the functional tests. CONCLUSION AND SIGNIFICANCE: These findings qualify gal-1 as a member of human trophoblast cell invasion machinery.
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