ArticleInternational journal of molecular sciences2011
Evaluation of internal reference genes for quantitative expression analysis by real-time PCR in ovine whole blood.
Article in International journal of molecular sciences, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
- Jejunal mucosal immune response in goats ten months afterThe veterinary quarterly · 2026Article
- Dynamics of antiretroviral restriction factor expression in sheep naturally infected with Small ruminant lentivirus (SRLV).Archives of virology · 2026Article
- Evaluation ofVaccines · 2025Article
- A comparison of normalization methods for the expression of genes associated with oxidative stress in the liver of sheep.BMC genomic data · 2025Article
- A novel quantitative real-time PCR with theVeterinarni medicina · 2024Article
- Analysis of the stability of the reference genes GAPDH, SDHA and RPL-19 in sheep from a semi-arid region infected by gastrointestinal nematodes.BMC veterinary research · 2023Article
- Evaluation of the innate immune response of caprine neutrophils against Mycobacterium avium subspecies paratuberculosis in vitro.Veterinary research · 2023Article
- Screening and stability analysis of reference genes in fasting caecotrophy model in rabbits.Molecular biology reports · 2022Article
- Characterization of MDCK cells and evaluation of their ability to respond to infectious and non-infectious stressors.Cytotechnology · 2020Article
- A Transcriptomic Study of the Tail Fat Deposition in Two Types of Hulun Buir Sheep According to Tail Size and Sex.Animals : an open access journal from MDPI · 2019Article
- Whole-Genome Resequencing Reveals Loci Associated With Thoracic Vertebrae Number in Sheep.Frontiers in genetics · 2019Article
- Identification of appropriate reference genes for local immune-related studies in Morada Nova sheep infected with Haemonchus contortus.Molecular biology reports · 2018Article
- A tissue-based approach to selection of reference genes for quantitative real-time PCR in a sheep osteoporosis model.BMC genomics · 2017Article
- Genome-wide analysis of circular RNAs in prenatal and postnatal pituitary glands of sheep.Scientific reports · 2017Article
- Article
- Evaluation of reference gene suitability for quantitative expression analysis by quantitative polymerase chain reaction in the mandibular condyle of sheep.Molecular medicine reports · 2015Article
- Design and evaluation of a unique SYBR Green real-time RT-PCR assay for quantification of five major cytokines in cattle, sheep and goats.BMC veterinary research · 2015Article
- Selection of reference genes for gene expression studies related to intramuscular fat deposition in Capra hircus skeletal muscle.PloS one · 2015Article
- Reference gene selection for quantitative PCR studies in sheep neutrophils.International journal of molecular sciences · 2013Article
- Selection of suitable reference genes for normalization of quantitative real-time PCR in cartilage tissue injury and repair in rabbits.International journal of molecular sciences · 2012Article
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10 authors.
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No grant is acknowledged in the PubMed record.
Abstract
The use of reference genes is commonly accepted as the most reliable approach to normalize qRT-PCR and to reduce possible errors in the quantification of gene expression. The most suitable reference genes in sheep have been identified for a restricted range of tissues, but no specific data on whole blood are available. The aim of this study was to identify a set of reference genes for normalizing qRT-PCR from ovine whole blood. We designed 11 PCR assays for commonly employed reference genes belonging to various functional classes and then determined their expression stability in whole blood samples from control and disease-stressed sheep. SDHA and YWHAZ were considered the most suitable internal controls as they were stably expressed regardless of disease status according to both geNorm and NormFinder software; furthermore, geNorm indicated SDHA/HPRT, YWHAZ/GAPDH and SDHA/YWHAZ as the best reference gene combinations in control, disease-stressed and combined sheep groups, respectively. Our study provides a validated panel of optimal control genes which may be useful for the identification of genes differentially expressed by qRT-PCR in a readily accessible tissue, with potential for discovering new physiological and disease markers and as a tool to improve production traits (e.g., by identifying expression Quantitative Trait Loci). An additional outcome of the study is a set of intron-spanning primer sequences suitable for gene expression experiments employing SYBR Green chemistry on other ovine tissues and cells.
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