ArticleThe Journal of biological chemistry2012
Phosphosite mapping of P-type plasma membrane H+-ATPase in homologous and heterologous environments.
Article in The Journal of biological chemistry, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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Who cites it
39 citing papers in PubMed, 70 citations in OpenAlex.
- Integrated proteomic analysis reveals adaptation mechanisms of paper mulberry seedling leaves to nitrogen stress.BMC plant biology · 2026Article
- Unveiling FERONIA receptor kinase-mediated cellular mechanisms with a small-molecule inhibitor.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Molecular tipping points in plant cell surface HQuantitative plant biology · 2025Review
- Phosphoregulation in the N-terminus of NRT2.1 affects nitrate uptake by controlling the interaction of NRT2.1 with NAR2.1 and kinase HPCAL1 in Arabidopsis.Journal of experimental botany · 2024Article
- Light-induced stomatal opening requires phosphorylation of the C-terminal autoinhibitory domain of plasma membrane HNature communications · 2024Article
- Anionic Phospholipids Stimulate the Proton Pumping Activity of the Plant Plasma Membrane P-Type HInternational journal of molecular sciences · 2023Article
- Receptor-like protein kinase BAK1 promotes K+ uptake by regulating H+-ATPase AHA2 under low potassium stress.Plant physiology · 2022Article
- Phosphorylation of the plasma membrane H+-ATPase AHA2 by BAK1 is required for ABA-induced stomatal closure in Arabidopsis.The Plant cell · 2022Article
- Proton and calcium pumping P-type ATPases and their regulation of plant responses to the environment.Plant physiology · 2021Review
- Protein Phosphorylation Response to Abiotic Stress in Plants.Methods in molecular biology (Clifton, N.J.) · 2021Review
- Tenuazonic acid from Stemphylium loti inhibits the plant plasma membrane HThe New phytologist · 2020Article
- Tissue-specific respiratory burst oxidase homolog-dependent H2O2 signaling to the plasma membrane H+-ATPase confers potassium uptake and salinity tolerance in Cucurbitaceae.Journal of experimental botany · 2019Article
- Ammonium and nitrate regulate NH4+ uptake activity of Arabidopsis ammonium transporter AtAMT1;3 via phosphorylation at multiple C-terminal sites.Journal of experimental botany · 2019Article
- Characterization of Hailey-Hailey Disease-mutants in presence and absence of wild type SPCA1 using Saccharomyces cerevisiae as model organism.Scientific reports · 2019Article
- The CaThe Plant cell · 2019Article
- Calcium-activated 14-3-3 proteins as a molecular switch in salt stress tolerance.Nature communications · 2019Article
- Early mannitol-triggered changes in the Arabidopsis leaf (phospho)proteome reveal growth regulators.Journal of experimental botany · 2018Article
- Article
- Blue Light Regulation of Stomatal Opening and the Plasma Membrane HPlant physiology · 2017Review
- A bioassay-guided fractionation system to identify endogenous small molecules that activate plasma membrane H+-ATPase activity in Arabidopsis.Journal of experimental botany · 2017Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Phosphorylation is an important posttranslational modification of proteins in living cells and primarily serves regulatory purposes. Several methods were employed for isolating phosphopeptides from proteolytically digested plasma membranes of Arabidopsis thaliana. After a mass spectrometric analysis of the resulting peptides we could identify 10 different phosphorylation sites in plasma membrane H(+)-ATPases AHA1, AHA2, AHA3, and AHA4/11, five of which have not been reported before, bringing the total number of phosphosites up to 11, which is substantially higher than reported so far for any other P-type ATPase. Phosphosites were almost exclusively (9 of 10) in the terminal regulatory domains of the pumps. The AHA2 isoform was subsequently expressed in the yeast Saccharomyces cerevisiae. The plant protein was phosphorylated at multiple sites in yeast, and surprisingly, seven of nine of the phosphosites identified in AHA2 were identical in the plant and fungal systems even though none of the target sequences in AHA2 show homology to proteins of the fungal host. These findings suggest an unexpected accessibility of the terminal regulatory domain of plasma membrane H(+)-ATPase to protein kinase action.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.