ArticleGenetics2012
Stringent analysis of gene function and protein-protein interactions using fluorescently tagged genes.
Article in Genetics, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 67 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
67 citing papers in PubMed.
- Genome-wide CRISPR knockout cell screening platform for the disease vector tick speciesbioRxiv : the preprint server for biology · 2026Article
- Bridges under construction: the dynamics of ring canal expansion during Drosophila oogenesis.Developmental biology · 2026Review
- Nanoparticle Interactions with the Blood Brain Barrier: Insights from Drosophila and Implications for Human Astrocyte Targeted Therapies.Neurochemical research · 2025Review
- A genome-engineered tool set for Drosophila TGF-β/BMP signaling studies.Development (Cambridge, England) · 2024Article
- Article
- Modular vector assembly enables rapid assessment of emerging CRISPR technologies.Cell genomics · 2024Article
- The Drosophila tumour suppressor Lgl and Vap33 activate the Hippo pathway through a dual mechanism.Journal of cell science · 2024Article
- Article
- Molecular symmetry breaking in the Frizzled-dependent planar polarity pathway.Current biology : CB · 2023Article
- Modular vector assembly enables rapid assessment of emerging CRISPR technologies.bioRxiv : the preprint server for biology · 2023Article
- Genetic and Epigenetic Regulation ofJournal of developmental biology · 2023Review
- Article
- Promoting validation and cross-phylogenetic integration in model organism research.Disease models & mechanisms · 2022Article
- Endocrine modulation of primary chemosensory neurons regulates Drosophila courtship behavior.PLoS genetics · 2022Article
- Ongoing transposition in cell culture reveals the phylogeny of diverse Drosophila S2 sublines.Genetics · 2022Article
- Protein visualization and manipulation ineLife · 2022Article
- Tagging Drosophila Proteins with Genetically Encoded Fluorophores.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Bioinformatic and cell-based tools for pooled CRISPR knockout screening in mosquitos.Nature communications · 2021Article
- A cis-regulatory element promoting increased transcription at low temperature in cultured ectothermic Drosophila cells.BMC genomics · 2021Article
- Characterizing the supercomplex association of photosynthetic complexes in cyanobacteria.Royal Society open science · 2021Article
7 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors.
Funding
Abstract
In Drosophila collections of green fluorescent protein (GFP) trap lines have been used to probe the endogenous expression patterns of trapped genes or the subcellular localization of their protein products. Here, we describe a method, based on nonoverlapping, highly specific, shRNA transgenes directed against GFP, that extends the utility of these collections to loss-of-function studies. Furthermore, we used a MiMIC transposon to generate GFP traps in Drosophila cell lines with distinct subcellular localization patterns, which will permit high-throughput screens using fluorescently tagged proteins. Finally, we show that fluorescent traps, paired with recombinant nanobodies and mass spectrometry, allow the study of endogenous protein complexes in Drosophila.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.