ArticleVirus genes2012
Real-time PCR assay for the discrimination and quantification of wheat and barley strains of Wheat dwarf virus.
Article in Virus genes, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 12 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
12 citing papers in PubMed.
- Article
- Development of Highly Efficient Resistance toInternational journal of molecular sciences · 2023Article
- Transcriptome Dynamics inViruses · 2023Article
- Asymmetric interactions between barley yellow dwarf virus -PAV and wheat dwarf virus in wheat.Frontiers in plant science · 2023Article
- Efficient Confirmation of Plant Viral Proteins and Identification of Specific Viral Strains by nanoLC-ESI-Q-TOF Using Single-Leaf-Tissue Samples.Pathogens (Basel, Switzerland) · 2020Article
- Development of loop-mediated isothermal amplification assay for rapid detection of genetically different wheat dwarf virus isolates.Molecular biology reports · 2020Article
- Functional Transcomplementation between Wheat Dwarf Virus Strains in Wheat and Barley.Viruses · 2019Article
- Recombinant expression of osmotin in barley improves stress resistance and food safety during adverse growing conditions.PloS one · 2019Article
- Monoclonal Antibody-Based Serological Detection Methods for Wheat Dwarf Virus.Virologica Sinica · 2018Article
- A sensitive method for the quantification of virion-sense and complementary-sense DNA strands of circular single-stranded DNA viruses.Scientific reports · 2014Article
- A one step real-time RT-PCR assay for the quantitation of Wheat yellow mosaic virus (WYMV).Virology journal · 2013Article
- Simultaneous detection and differentiation of Rice black streaked dwarf virus (RBSDV) and Southern rice black streaked dwarf virus (SRBSDV) by duplex real time RT-PCR.Virology journal · 2013Article
Corrections and comments
- Erratum issued
Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A rapid method for detection, discrimination and quantification of wheat and barley strains of wheat dwarf virus (WDV) was successfully developed. The sensitivity of quantification of the wheat and barley strains of WDV ranged from an average of 1.2 × 10(7)-1.2 × 10(2) and from an average of 1.4 × 10(7)-1.4 × 10(4) copies of viral genome, respectively. These standard serial dilutions were applied to plant and vector tissues for virus titer calculations. Both strains of WDV were clearly discriminated by specific probes and melting curve analysis. Both TaqMan(®) and SYBR(®) Green technologies provided accurate and reliable methods for monitoring, detection, discrimination, and quantification of WDV.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.