ArticlePlant physiology2012
The function of RH22, a DEAD RNA helicase, in the biogenesis of the 50S ribosomal subunits of Arabidopsis chloroplasts.
Article in Plant physiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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Who cites it
38 citing papers in PubMed, 82 citations in OpenAlex.
- The Regulatory Roles of RNA-Binding Proteins in Plant Salt Stress Response.Plants (Basel, Switzerland) · 2025Review
- Novel resources to investigate leaf plasmodesmata formation in CThe Plant journal : for cell and molecular biology · 2024Article
- Phenotypic and microarray analysis reveals salinity stress-induced oxidative tolerance in transgenic rice expressing a DEAD-box RNA helicase, OsDB10.Plant molecular biology · 2023Article
- Article
- Functions and mechanisms of RNA helicases in plants.Journal of experimental botany · 2023Review
- Stromal Protein Chloroplast Development and Biogenesis1 Is Essential for Chloroplast Development and Biogenesis inFrontiers in plant science · 2022Article
- A PPR Protein ACM1 Is Involved in Chloroplast Gene Expression and Early Plastid Development inInternational journal of molecular sciences · 2021Article
- The res (restored cell structure by salinity) tomato mutant reveals the role of the DEAD-box RNA helicase SlDEAD39 in plant development and salt response.Plant, cell & environment · 2020Article
- Roles of Organellar RNA-Binding Proteins in Plant Growth, Development, and Abiotic Stress Responses.International journal of molecular sciences · 2020Review
- Chloroplast-to-nucleus retrograde signalling controls intercellular trafficking via plasmodesmata formation.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2020Article
- The Arabidopsis Protein CGL20 Is Required for Plastid 50S Ribosome Biogenesis.Plant physiology · 2020Article
- PDM4, a Pentatricopeptide Repeat Protein, Affects Chloroplast Gene Expression and Chloroplast Development inFrontiers in plant science · 2020Article
- Genetic mapping of green curd gene Gr in cauliflower.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2020Article
- Article
- WSL6 encoding an Era-type GTP-binding protein is essential for chloroplast development in rice.Plant molecular biology · 2019Article
- An RNA Chaperone-Like Protein Plays Critical Roles in Chloroplast mRNA Stability and Translation in Arabidopsis and Maize.The Plant cell · 2019Article
- Rice OsRH58, a chloroplast DEAD-box RNA helicase, improves salt or drought stress tolerance in Arabidopsis by affecting chloroplast translation.BMC plant biology · 2019Article
- The DEAD-box RNA Helicase RH50 Is a 23S-4.5S rRNA Maturation Factor that Functionally Overlaps with the Plastid Signaling Factor GUN1.Plant physiology · 2018Article
- Function of Plant DExD/H-Box RNA Helicases Associated with Ribosomal RNA Biogenesis.Frontiers in plant science · 2018Review
- Lethal albinic seedling, encoding a threonyl-tRNA synthetase, is involved in development of plastid protein synthesis system in rice.Plant cell reports · 2017Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The chloroplast ribosome is a large and dynamic ribonucleoprotein machine that is composed of the 30S and 50S subunits. Although the components of the chloroplast ribosome have been identified in the last decade, the molecular mechanisms driving chloroplast ribosome biogenesis remain largely elusive. Here, we show that RNA helicase 22 (RH22), a putative DEAD RNA helicase, is involved in chloroplast ribosome assembly in Arabidopsis (Arabidopsis thaliana). A loss of RH22 was lethal, whereas a knockdown of RH22 expression resulted in virescent seedlings with clear defects in chloroplast ribosomal RNA (rRNA) accumulation. The precursors of 23S and 4.5S, but not 16S, rRNA accumulated in rh22 mutants. Further analysis showed that RH22 was associated with the precursors of 50S ribosomal subunits. These results suggest that RH22 may function in the assembly of 50S ribosomal subunits in chloroplasts. In addition, RH22 interacted with the 50S ribosomal protein RPL24 through yeast two-hybrid and pull-down assays, and it was also bound to a small 23S rRNA fragment encompassing RPL24-binding sites. This action of RH22 may be similar to, but distinct from, that of SrmB, a DEAD RNA helicase that is involved in the ribosomal assembly in Escherichia coli, which suggests that DEAD RNA helicases and rRNA structures may have coevolved with respect to ribosomal assembly and function.
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