ArticleJournal of clinical microbiology2012
Identification of infectious agents in onychomycoses by PCR-terminal restriction fragment length polymorphism.
Article in Journal of clinical microbiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers, 1 of them a synthesis that pooled it.
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Who cites it
17 citing papers in PubMed, 1 synthesis or guideline pooled it, 55 citations in OpenAlex.
- Oral antifungal medication for toenail onychomycosis.The Cochrane database of systematic reviews · 2017Pooled it
- A six-year retrospective study on the causative agents of onychomycosis in China: the emergence of dematiaceous fungi.Frontiers in microbiology · 2025Article
- Detection of Pan-Dermatophytes and Trichophyton rubrum Using Recombinase Polymerase Amplification-Lateral Flow Dipstick Assay.Mycopathologia · 2024Article
- Molecular identification of yeast communities isolated from nail specimens by PCR-RFLP and PCR-FSP methods.Current medical mycology · 2024Article
- Survey on Dermatophytes Isolated from Animals in Switzerland in the Context of the Prevention of Zoonotic Dermatophytosis.Journal of fungi (Basel, Switzerland) · 2023Article
- Identification of Dermatophyte and Non-Dermatophyte Agents in Onychomycosis by PCR and DNA Sequencing-A Retrospective Comparison of Diagnostic Tools.Journal of fungi (Basel, Switzerland) · 2022Article
- Updated Perspectives on the Diagnosis and Management of Onychomycosis.Clinical, cosmetic and investigational dermatology · 2022Review
- Recent trends in rapid diagnostic techniques for dermatophytosis.International journal of veterinary science and medicine · 2020Review
- Epidemiology of Dermatophytoses in Switzerland According to a Survey of Dermatophytes Isolated in Lausanne between 2001 and 2018.Journal of fungi (Basel, Switzerland) · 2020Article
- Recent Findings in Onychomycosis and Their Application for Appropriate Treatment.Journal of fungi (Basel, Switzerland) · 2019Review
- Terbinafine Resistance of Trichophyton Clinical Isolates Caused by Specific Point Mutations in the Squalene Epoxidase Gene.Antimicrobial agents and chemotherapy · 2017Article
- Diagnosis of Dermatophytosis Using Molecular Biology.Mycopathologia · 2017Review
- Production of Fusaric Acid by Fusarium spp. in Pure Culture and in Solid Medium Co-Cultures.Molecules (Basel, Switzerland) · 2016Article
- Development and Evaluation of a Novel Real-Time PCR for Pan-Dermatophyte Detection in Nail Specimens.Mycopathologia · 2016Article
- [Molecular biological detection of dermatophytes in clinical samples when onychomycosis or tinea pedis is suspected. A prospective study comparing conventional dermatomycological diagnostics and polymerase chain reaction].Der Hautarzt; Zeitschrift fur Dermatologie, Venerologie, und verwandte Gebiete · 2013Article
- [Skin infections caused by Fusarium].Der Hautarzt; Zeitschrift fur Dermatologie, Venerologie, und verwandte Gebiete · 2012Article
- A Pilot Study for the Evaluation of PCR as a Diagnostic Tool in Patients with Suspected Dermatophytoses.Indian dermatology online journalArticle
Corrections and comments
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Authors and funding
11 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A fast and reliable assay for the identification of dermatophyte fungi and nondermatophyte fungi (NDF) in onychomycosis is essential, since NDF are especially difficult to cure using standard treatment. Diagnosis is usually based on both direct microscopic examination of nail scrapings and macroscopic and microscopic identification of the infectious fungus in culture assays. In the last decade, PCR assays have been developed for the direct detection of fungi in nail samples. In this study, we describe a PCR-terminal restriction fragment length polymorphism (TRFLP) assay to directly and routinely identify the infecting fungi in nails. Fungal DNA was easily extracted using a commercial kit after dissolving nail fragments in an Na(2)S solution. Trichophyton spp., as well as 12 NDF, could be unambiguously identified by the specific restriction fragment size of 5'-end-labeled amplified 28S DNA. This assay enables the distinction of different fungal infectious agents and their identification in mixed infections. Infectious agents could be identified in 74% (162/219) of cases in which the culture results were negative. The PCR-TRFLP assay described here is simple and reliable. Furthermore, it has the possibility to be automated and thus routinely applied to the rapid diagnosis of a large number of clinical specimens in dermatology laboratories.
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