ArticleCytometry. Part A : the journal of the International Society for Analytical Cytology2012
Multispectral imaging flow cytometry reveals distinct frequencies of γ-H2AX foci induction in DNA double strand break repair defective human cell lines.
Article in Cytometry. Part A : the journal of the International Society for Analytical Cytology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
23 citing papers in PubMed, 47 citations in OpenAlex.
- Mitochondrial DNA is a sensitive surrogate and oxidative stress target in oral cancer cells.PloS one · 2024Article
- Development of high-throughput systems for biodosimetry.Radiation protection dosimetry · 2023Article
- The Imaging Flow Cytometry-Based Cytokinesis-Block MicroNucleus (CBMN) Assay.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Transcription-associated DNA DSBs activate p53 during hiPSC-based neurogenesis.Scientific reports · 2022Article
- Imaging flow cytometry: a primer.Nature reviews. Methods primers · 2022Article
- Deepometry, a framework for applying supervised and weakly supervised deep learning to imaging cytometry.Nature protocols · 2021Article
- Ionizing Radiation Protein Biomarkers in Normal Tissue and Their Correlation to Radiosensitivity: A Systematic Review.Journal of personalized medicine · 2021Review
- Article
- Microwaves from mobile phone induce reactive oxygen species but not DNA damage, preleukemic fusion genes and apoptosis in hematopoietic stem/progenitor cells.Scientific reports · 2019Article
- Radio-selective effects of a natural occurring muscle-derived dipeptide in A549 and normal cell lines.Scientific reports · 2019Article
- Imaging Flow Cytometry Quantifies Neural Genome Dynamics.Cytometry. Part A : the journal of the International Society for Analytical Cytology · 2019Article
- Automation of the in vitro micronucleus assay using the ImagestreamCytometry. Part A : the journal of the International Society for Analytical Cytology · 2018Article
- Phosphorylated fraction of H2AX as a measurement for DNA damage in cancer cells and potential applications of a novel assay.PloS one · 2017Article
- The PARP-1 inhibitor Olaparib suppresses BRCA1 protein levels, increases apoptosis and causes radiation hypersensitivity in BRCA1Journal of Cancer · 2017Article
- The Application of Imaging Flow Cytometry to High-Throughput Biodosimetry.Genome integrity · 2017Review
- An open-source solution for advanced imaging flow cytometry data analysis using machine learning.Methods (San Diego, Calif.) · 2017Article
- Lentivirus-meditated frataxin gene delivery reverses genome instability in Friedreich ataxia patient and mouse model fibroblasts.Gene therapy · 2016Article
- A flow cytometry-based method to simplify the analysis and quantification of protein association to chromatin in mammalian cells.Nature protocols · 2015Article
- Enhanced γ-H2AX DNA damage foci detection using multimagnification and extended depth of field in imaging flow cytometry.Cytometry. Part A : the journal of the International Society for Analytical Cytology · 2015Article
- Improved quantitative analysis of primary bone marrow megakaryocytes utilizing imaging flow cytometry.Cytometry. Part A : the journal of the International Society for Analytical Cytology · 2014Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The measurement of γ-H2AX foci induction in cells provides a sensitive and reliable method for the quantitation of DNA damage responses in a variety of cell types. Accurate and rapid methods to conduct such observations are desirable. In this study, we have employed the novel technique of multispectral imaging flow cytometry to compare the induction and repair of γ-H2AX foci in three human cell types with different capacities for the repair of DNA double strand breaks (DSB). A repair normal fibroblast cell line MRC5-SV1, a DSB repair defective ataxia telangiectasia (AT5BIVA) cell line, and a DNA-PKcs deficient cell line XP14BRneo17 were exposed to 2 Gy gamma radiation from a (60)Cobalt source. Thirty minutes following exposure, we observed a dramatic induction of foci in the nuclei of these cells. After 24 hrs, there was a predictable reduction on the number of foci in the MRC5-SV1 cells, consistent with the repair of DNA DSB. In the AT5BIVA cells, persistence of the foci over a 24-hr period was due to the failure in the repair of DNA DSB. However, in the DNA-PKcs defective cells (XP14BRneo17), we observed an intermediate retention of foci in the nuclei indicative of partial repair of DNA DSB. In summary, the application of imaging flow cytometry has permitted an evaluation of foci in a large number of cells (20,000) for each cell line at each time point. This provides a novel method to determine differences in repair kinetics between different cell types. We propose that imaging flow cytometry provides an alternative platform for accurate automated high through-put analysis of foci induction in a variety of cell types.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.