ArticleThe Journal of biological chemistry2012
Vitamin C deficiency activates the purine nucleotide cycle in zebrafish.
Article in The Journal of biological chemistry, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
42 citing papers in PubMed, 65 citations in OpenAlex.
- Xanthohumol Alters Gut Microbiota Metabolism and Bile Acid Dynamics in Gastrointestinal Simulation Models of Eubiotic and Dysbiotic States.International journal of molecular sciences · 2025Article
- The first report of AMP-deaminase activity in skeletal muscles of Lates calcarifer and its stunning adaptation to ammonia poisoning.Fish physiology and biochemistry · 2025Article
- Learning genotype-phenotype associations from gaps in multi-species sequence alignments.Briefings in bioinformatics · 2024Article
- Mechanism of vitamin C alleviating the immunotoxicity of 17α-methyltestosterone in Carassius auratus.BMC genomics · 2024Article
- Deciphering the enigma of the function of alpha-tocopherol as a vitamin.Free radical biology & medicine · 2024Article
- Identification of biological signatures of cruciferous vegetable consumption utilizing machine learning-based global untargeted stable isotope traced metabolomics.Frontiers in nutrition · 2024Article
- Chronic Vitamin E Deficiency Dysregulates Purine, Phospholipid, and Amino Acid Metabolism in Aging Zebrafish Skeletal Muscle.Antioxidants (Basel, Switzerland) · 2023Article
- Interplay between Cruciferous Vegetables and the Gut Microbiome: A Multi-Omic Approach.Nutrients · 2022Article
- Alteration of Gut Microbiome and Correlated Amino Acid Metabolism Contribute to Hyperuricemia and Th17-Driven Inflammation inFrontiers in immunology · 2022Article
- Gene Expression of CRAL_TRIO Family Proteins modulated by Vitamin E Deficiency in Zebrafish (Danio Rerio).The Journal of nutritional biochemistry · 2021Article
- Vitamin E: necessary nutrient for neural development and cognitive function.The Proceedings of the Nutrition Society · 2021Article
- RedEfish: Generation of the Polycistronic mScarlet: GSG-T2A: Ttpa Zebrafish Line.Antioxidants (Basel, Switzerland) · 2021Article
- Transkingdom interactions between Lactobacilli and hepatic mitochondria attenuate western diet-induced diabetes.Nature communications · 2021Article
- Growth and Antioxidant-Related Effects of the Reestablished Ascorbic Acid Pathway in Zebrafish (Frontiers in physiology · 2021Article
- Recent Application of Zebrafish Models in Atherosclerosis Research.Frontiers in cell and developmental biology · 2021Review
- Vitamin C Activates the Folate-Mediated One-Carbon Cycle in C2C12 Myoblasts.Antioxidants (Basel, Switzerland) · 2020Article
- Nitrate and nitrite exposure leads to mild anxiogenic-like behavior and alters brain metabolomic profile in zebrafish.PloS one · 2020Article
- Treatment with Nitrate, but Not Nitrite, Lowers the Oxygen Cost of Exercise and Decreases Glycolytic Intermediates While Increasing Fatty Acid Metabolites in Exercised Zebrafish.The Journal of nutrition · 2019Article
- Vitamin C-dependent lysine demethylase 6 (KDM6)-mediated demethylation promotes a chromatin state that supports the endothelial-to-hematopoietic transition.The Journal of biological chemistry · 2019Article
- Current basis and future directions of zebrafish nutrigenomics.Genes & nutrition · 2019Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors at 2 institutions in 2 countries.
Funding
Abstract
Vitamin C (ascorbic acid, AA) is a cofactor for many important enzymatic reactions and a powerful antioxidant. AA provides protection against oxidative stress by acting as a scavenger of reactive oxygen species, either directly or indirectly by recycling of the lipid-soluble antioxidant, α-tocopherol (vitamin E). Only a few species, including humans, guinea pigs, and zebrafish, cannot synthesize AA. Using an untargeted metabolomics approach, we examined the effects of α-tocopherol and AA deficiency on the metabolic profiles of adult zebrafish. We found that AA deficiency, compared with subsequent AA repletion, led to oxidative stress (using malondialdehyde production as an index) and to major increases in the metabolites of the purine nucleotide cycle (PNC): IMP, adenylosuccinate, and AMP. The PNC acts as a temporary purine nucleotide reservoir to keep AMP levels low during times of high ATP utilization or impaired oxidative phosphorylation. The PNC promotes ATP regeneration by converting excess AMP into IMP, thereby driving forward the myokinase reaction (2ADP → AMP + ATP). On the basis of this finding, we investigated the activity of AMP deaminase, the enzyme that irreversibly deaminates AMP to form IMP. We found a 47% increase in AMP deaminase activity in the AA-deficient zebrafish, complementary to the 44-fold increase in IMP concentration. These results suggest that vitamin C is crucial for the maintenance of cellular energy metabolism.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.