ArticleNucleic acids research2012
Metal-induced DNA translocation leads to DNA polymerase conformational activation.
Article in Nucleic acids research, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
What it found
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Who cites it
27 citing papers in PubMed, 35 citations in OpenAlex.
- Changes of serum trace elements in early stage trauma and its correlation with injury severity score.Medicine · 2018Trial
- Coordinated Residue Motions at the Enzyme-Substrate Interface Promote DNA Translocation in Polymerases.Journal of the American Chemical Society · 2025Article
- Structural and Molecular Kinetic Features of Activities of DNA Polymerases.International journal of molecular sciences · 2022Review
- The comparative effects of manganese nanoparticles and their counterparts (bulk and ionic) inFrontiers in plant science · 2022Article
- Visualizing Rev1 catalyze protein-template DNA synthesis.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Lysines in the lyase active site of DNA polymerase β destabilize nonspecific DNA binding, facilitating searching and DNA gap recognition.The Journal of biological chemistry · 2020Article
- DNA polymerase β nucleotide-stabilized template misalignment fidelity depends on local sequence context.The Journal of biological chemistry · 2020Article
- I260Q DNA polymerase β highlights precatalytic conformational rearrangements critical for fidelity.Nucleic acids research · 2018Article
- Transitions in DNA polymerase β μs-ms dynamics related to substrate binding and catalysis.Nucleic acids research · 2018Article
- Modulating the DNA polymerase β reaction equilibrium to dissect the reverse reaction.Nature chemical biology · 2017Article
- Structures of DNA Polymerase Mispaired DNA Termini Transitioning to Pre-catalytic Complexes Support an Induced-Fit Fidelity Mechanism.Structure (London, England : 1993) · 2016Article
- Article
- Insertion of oxidized nucleotide triggers rapid DNA polymerase opening.Nucleic acids research · 2016Article
- Modulation of DNA Polymerase Noncovalent Kinetic Transitions by Divalent Cations.The Journal of biological chemistry · 2016Article
- Substrate-induced DNA polymerase β activation.The Journal of biological chemistry · 2014Article
- Structure and mechanism of DNA polymerase β.Biochemistry · 2014Article
- Metal-dependent conformational activation explains highly promutagenic replication across O6-methylguanine by human DNA polymerase β.Journal of the American Chemical Society · 2014Article
- Article
- Optimal and variant metal-ion routes in DNA polymerase β's conformational pathways.Journal of the American Chemical Society · 2014Article
- Structural basis for promutagenicity of 8-halogenated guanine.The Journal of biological chemistry · 2014Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
Abstract
Binding of the catalytic divalent ion to the ternary DNA polymerase β/gapped DNA/dNTP complex is thought to represent the final step in the assembly of the catalytic complex and is consequently a critical determinant of replicative fidelity. We have analyzed the effects of Mg(2+) and Zn(2+) on the conformational activation process based on NMR measurements of [methyl-(13)C]methionine DNA polymerase β. Unexpectedly, both divalent metals were able to produce a template base-dependent conformational activation of the polymerase/1-nt gapped DNA complex in the absence of a complementary incoming nucleotide, albeit with different temperature thresholds. This conformational activation is abolished by substituting Glu295 with lysine, thereby interrupting key hydrogen bonds necessary to stabilize the closed conformation. These and other results indicate that metal-binding can promote: translocation of the primer terminus base pair into the active site; expulsion of an unpaired pyrimidine, but not purine, base from the template-binding pocket; and motions of polymerase subdomains that close the active site. We also have performed pyrophosphorolysis studies that are consistent with predictions based on these results. These findings provide new insight into the relationships between conformational activation, enzyme activity and polymerase fidelity.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.